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JBC, Vol. 252, Issue 9, 2860-2865, May, 1977

Detection of multiple forms of rat liver ornithine decarboxylase

M. F. Obenrader and W. F. Prouty

Rat liver ornithine decarboxylase induced by injection of thioacetamide has been separated into at least two fractions by covalent chromatography on an activated thiol-Sepharose 4B column. The two major fractions could be distinguished by ion exchange chromatography and electrophoresis on acrylamide gels. In addition, the two forms displayed different Km values for ornithine. Although the two forms are separable, they display identical antigenic properties, pH optima, and they appear to be the same molecular size. The biological significance or the relationship between multiple forms of ornithine decarboxylase is not understood.
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Copyright © 1977 by the American Society for Biochemistry and Molecular Biology.