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JBC, Vol. 253, Issue 12, 4310-4318, Jun, 1978
J. A. Idell-Wenger, L. W. Grotyohann and J. R. Neely
The distribution of coenzyme A and carnitine between the mitochondrial and
cytosolic compartments was determined in rat heart ventricular muscle. The
CoA and carnitine levels of homogenate, mitochondrial, and
postmitochondrial fractions were determined in nonperfused hearts and in
hearts that were perfused under control and ischemic conditions. Using the
mitochondrial marker enzymes, citrate synthase and cytochrome c oxidase,
the cellular content of mitochondrial protein was determined to be 53 +/-
1.0 (nonperfused), 53.5 +/- 1.5 (control), and 58.1 +/- 2.2 (ischemic) mg/g
of wet heart muscle. These values were used to calculate the contribution
of the CoA and carnitine located in the mitochondrial compartment to the
total cellular levels of CoA and carnitine. Under both control and ischemic
conditions, approximately 95% of the cellular CoA was mitochondrial. The
percentage of the total cellular carnitine associated with the mitochondria
increased from 8 to 9% in nonperfused and control hearts to 25% during
ischemia, indicating that a net transfer of carnitine occurred from the
cytosol to the mitochondrial matrix.
Coenzyme A and carnitine distribution in normal and ischemic hearts
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