| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
JBC, Vol. 253, Issue 12, 4419-4425, Jun, 1978
A. Duran and E. Cabib
Chitin synthetase was solubilized with digitonin from a particulate yeast
fraction. The solubilized enzyme, which did not sediment at 200,000 X g and
emerged after the void volume in a Sepharose 6B column, was active only
after treatment with a protease. This confirms that chitin synthetase
exists in the plasma membrane as a zymogen and that initiation of the
chitin septum occurs by localized activation of the enzyme. By differential
extraction with sodium cholate and digitonin, followed by chromatography on
Sepharose 6B, a 20-fold purification of the enzyme was achieved with
respect to the crude particles. The purified enzyme showed a requirement
for a phospholipid; phosphatidylserine and lysophosphatidylserine were the
best activators. Unsaturated fatty acids strongly inhibited synthetase
activity, whereas their saturated counterparts were inert. The solubilized
enzyme catalyzed the formation of insoluble chitin in the absence of added
primer. The synthetic polysaccharide was examined by electron microscopy
and found to consist of lozenge-shaped particles about 60 nm long and 10 nm
wide.
Solubilization and partial purification of yeast chitin synthetase. Confirmation of the zymogenic nature of the enzyme
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. D. Karr and E. L. Jarroll Cyst wall synthase: N-acetylgalactosaminyltransferase activity is induced to form the novel N-acetylgalactosamine polysaccharide in the Giardia cyst wall Microbiology, May 1, 2004; 150(5): 1237 - 1243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Merzendorfer and L. Zimoch Chitin metabolism in insects: structure, function and regulation of chitin synthases and chitinases J. Exp. Biol., December 15, 2003; 206(24): 4393 - 4412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Bulik, M. Olczak, H. A. Lucero, B. C. Osmond, P. W. Robbins, and C. A. Specht Chitin Synthesis in Saccharomyces cerevisiae in Response to Supplementation of Growth Medium with Glucosamine and Cell Wall Stress Eukaryot. Cell, October 1, 2003; 2(5): 886 - 900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Mergaert, W. D'Haeze, D. Geelen, D. Promé, M. Van Montagu, R. Geremia, J.-C. Promé, and M. Holsters Biosynthesis of Azorhizobium caulinodans Nod Factors J. Biol. Chem., December 8, 1995; 270(49): 29217 - 29223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nagahashi, M. Sudoh, N. Ono, R. Sawada, E. Yamaguchi, Y. Uchida, T. Mio, M. Takagi, M. Arisawa, and H. Yamada-Okabe Characterization of Chitin Synthase 2 of Saccharomyces cerevisiae J. Biol. Chem., June 9, 1995; 270(23): 13961 - 13967. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Kalebic, S Garbisa, B Glaser, and L. Liotta Basement membrane collagen: degradation by migrating endothelial cells Science, July 15, 1983; 221(4607): 281 - 283. [Abstract] [PDF]
|
|
|
|
|
|
A Duran, E Cabib, and B Bowers Chitin synthetase distribution on the yeast plasma membrane Science, January 26, 1979; 203(4378): 363 - 365. [Abstract] [PDF]
|
|
|
|
|
|
M. Sudoh, T. Yamazaki, K. Masubuchi, M. Taniguchi, N. Shimma, M. Arisawa, and H. Yamada-Okabe Identification of a Novel Inhibitor Specific to the Fungal Chitin Synthase. INHIBITION OF CHITIN SYNTHASE 1 ARRESTS THE CELL GROWTH, BUT INHIBITION OF CHITIN SYNTHASE 1 AND 2 IS LETHAL IN THE PATHOGENIC FUNGUS CANDIDA ALBICANS J. Biol. Chem., October 13, 2000; 275(42): 32901 - 32905. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
