HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Oppenheimer, N. J. Search for Related Content PubMed PubMed Citation Articles by Oppenheimer, N. J. Social Bookmarking                      What's this?

JBC, Vol. 253, Issue 14, 4907-4910, Jul, 1978

Structural determination and stereospecificity of the choleragen-catalyzed reaction of NAD+ with guanidines

N. J. Oppenheimer

The products of the choleragen-catalyzed reaction of NAD+ with guanidine HCl or L-arginine have been isolated by high performance liquid chromatography. Analysis of 1H NMR spectra obtained at 360 MHz establishes the structure of the isolated products to be adenosine diphosphoribosyl guanidiniums present as 1:1 mixtures of alpha and beta anomeric forms. Direct observation of the choleragen-catalyzed reaction of NAD+ with L-arginine by 1H NMR spectroscopy establishes that choleragen synthesizes an alpha anomeric linkage that is subject to subsequent anomerization. The stereospecificity of the reaction provides further evidence that choleragen behaves as an enzyme and may be related mechanistically to other ADP-ribosyltransferases.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Kato, J. Zhu, C. Liu, and J. Moss Enhanced Sensitivity to Cholera Toxin in ADP-Ribosylarginine Hydrolase-Deficient Mice Mol. Cell. Biol., August 1, 2007; 27(15): 5534 - 5543. [Abstract] [Full Text] [PDF]

				A. R. Morrison, J. Moss, L. A. Stevens, J. E. Evans, C. Farrell, E. Merithew, D. G. Lambright, D. L. Greiner, J. P. Mordes, A. A. Rossini, et al. ART2, a T Cell Surface Mono-ADP-ribosyltransferase, Generates Extracellular Poly(ADP-ribose) J. Biol. Chem., November 3, 2006; 281(44): 33363 - 33372. [Abstract] [Full Text] [PDF]

				A. K. Ganesan, T. S. Vincent, J. C. Olson, and J. T. Barbieri Pseudomonas aeruginosa Exoenzyme S Disrupts Ras-mediated Signal Transduction by Inhibiting Guanine Nucleotide Exchange Factor-catalyzed Nucleotide Exchange J. Biol. Chem., July 30, 1999; 274(31): 21823 - 21829. [Abstract] [Full Text] [PDF]

				J. Moss, L. A. Stevens, E. Cavanaugh, I. J. Okazaki, R. Bortell, T. Kanaitsuka, J. P. Mordes, D. L. Greiner, and A. A. Rossini Characterization of Mouse Rt6.1 NAD:Arginine ADP-ribosyltransferase J. Biol. Chem., February 14, 1997; 272(7): 4342 - 4346. [Abstract] [Full Text] [PDF]

				D. Cervantes-Laurean, P. T. Loflin, D. E. Minter, E. L. Jacobson, and M. K. Jacobson Protein Modification by ADP-ribose via Acid-labile Linkages J. Biol. Chem., April 7, 1995; 270(14): 7929 - 7936. [Abstract] [Full Text] [PDF]

				T. Takamura-Enya, M. Watanabe, Y. Totsuka, T. Kanazawa, Y. Matsushima-Hibiya, K. Koyama, T. Sugimura, and K. Wakabayashi Mono(ADP-ribosyl)ation of 2'-deoxyguanosine residue in DNA by an apoptosis-inducing protein, pierisin-1, from cabbage butterfly PNAS, October 23, 2001; 98(22): 12414 - 12419. [Abstract] [Full Text] [PDF]