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JBC, Vol. 254, Issue 11, 4305-4308, Jun, 1979

Heme-associated subunit complex of cytochrome c oxidase identified by a new two-dimensional gel electrophoresis

J. A. Freedman, R. P. Tracy and S. H. Chan

A Novel two-dimensional system (isoelectric focusing in the first dimension and sodium dodecyl sulfate-urea electrophoresis in the second) was used to determine the pI values of beef heart subunits of cytochrome c oxidase. The results identified a heme-associated complex of Subunits I, II, and IV. Conditions mitigate against migration of free heme.
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Copyright © 1979 by the American Society for Biochemistry and Molecular Biology.