HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Adams, S. L. Articles by Pastan, I. Search for Related Content PubMed PubMed Citation Articles by Adams, S. L. Articles by Pastan, I. Social Bookmarking                      What's this?

JBC, Vol. 254, Issue 12, 4935-4938, Jun, 1979

Use of recombinant plasmids to characterize collagen RNAs in normal and transformed chick embryo fibroblasts

S. L. Adams, J. C. Alwine, B. de Crombrugghe and I. Pastan

Two recombinant plasmids containing chick collagen DNA sequences have been used to characterize messenger RNAs for pro-alpha1 (type I) and pro-alpha2 collagen. Poly(A)-containing RNA from chick embryo calvaria and long bones, tissues which are very active in collagen synthesis, were electrophoresed on agarose gels containing methylmercuric hydroxide and transferred to diazobenzyloxymethyl paper; these covalently bound RNAs were hybridized to 32P-labeled pro-alpha1 or pro-alpha2 collagen DNA sequences derived from the recombinant plasmids. The pro-alpha1 collagen probe identified two RNAs, a major species of 5000 bases and a minor species of 7100 bases; the pro-alpha2 collagen probe hybridized to a major species very similar in size to the pro-alpha1 mRNA, about 5200 bases, and a minor species of 5700 bases. It is possible that the 7100 and 5700 base RNAs represent precursors of pro-alpha1 and pro-alpha2 collagen mRNA, respectively. When similar hybridization experiments were performed with RNA from chick embryo fibroblasts, both the pro-alpha1 and pro-alpha2 collagen mRNAs were observed, as well as their corresponding larger species. With RNAs from fibroblasts transformed by Rous sarcoma virus, however, the levels of all RNA species which hybridized with the pro-alpha1 and pro-alpha2 collagen DNA probes were significantly reduced.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				H. Inoue, J. Pan, and A. Hakura Suppression of v-src Transformation by the drs Gene J. Virol., March 1, 1998; 72(3): 2532 - 2537. [Abstract] [Full Text] [PDF]

				G. Vogeli, H. Ohkubo, V. E. Avvedimento, M. Sullivan, Y. Yamada, M. Mudryj, I. Pastan, and B. De Crombrugghe A Repetitive Structure in the Chick {alpha}2-Collagen Gene Cold Spring Harb Symp Quant Biol, January 1, 1981; 45(0): 777 - 783. [Abstract] [PDF]