JBC, Vol. 254, Issue 13, 5832-5837, Jul, 1979
The amino acid sequence of fragment A, an enzymically active fragment of diphtheria toxin. II. The cyanogen bromide peptides
R. E. Drazin, R. J. Collier, L. C. Williams and R. J. DeLange
Cyanogen bromide cleavage of Fragment A from diphtheria toxin at the four
methionines present in each molecule resulted in five major peptides which
were isolated and studied by sequence methods. These five peptides of 4,
11, 14, 63, and 101 residues account for all 193 residues in Fragment A and
provide overlaps for the tryptic peptides from the maleylated protein. Two
additional peptides were isolated and shown to be shorter forms (8 and 10
residues) of the COOH-terminal cyanogen bromide peptide (11 residues).