JBC, Vol. 254, Issue 19, 9860-9866, Oct, 1979
Specific transcription in chicken liver chromatin by endogenous RNA polymerase II. Comparison of an estrogen-inducible gene with a constitutively expressed gene
K. P. Mullinix, M. B. Meyers, J. L. Christmann, R. G. Deeley, J. I. Gordon and R. F. Goldberger
We have developed a system for the in vitro transcription of specific genes
in rooster liver chromatin by endogenous RNA polymerase II that maintains
the specificity of transcription in vivo. Radioactive transcripts
synthesized in vitro were identified and quantitated by hybridization to a
vast excess of cloned cDNA. The cDNA preparations employed corresponded to
vitellogenin mRNA, the synthesis of which is responsive to estrogen
stimulation in vivo, and chicken serum albumin mRNA, the synthesis of which
is not significantly affected by estrogen stimulation in vivo. Comparing
the pattern of transcription of the albumin and vitellogenin genes in
chromatin from the liver of the normal rooster with the pattern in
chromatin from the liver of the estrogen-stimulated rooster, we found that
prior estrogen treatment of the rooster is attended by a slight decrease in
the differential rate of transcription of the albumin gene and
approximately a 10-fold increase in the differential rate of transcription
of the vitellogenin gene. Because this pattern of transcription reflects
the estrogen-induced changes in transcription observed in vivo, chromatin
preparations from the livers of normal and estrogen-stimulated roosters can
be used to investigate regulation of specific gene transcription at the
molecular level in vitro.
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