JBC Transcription and Nuclear Factor Monoclonals

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J. Biol. Chem., Vol. 255, Issue 10, 4511-4515, 05, 1980

A nonamer of histones in chromatin

TL Reudelhuber, T Boulikas and WT Garrard

The results of dimethyl suberimidate cross-linking experiments demonstrate that histone H1 can be cross-linked quantitatively to the histone octamer of mononucleosomes to yield a histone nonamer. By combining our cross-linking procedure with two-dimensional electrophoretic techniques, we show further that it is possible to trace the nucleosomal origins of a spectrum of cross-linked chromatin products.
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