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J. Biol. Chem., Vol. 255, Issue 11, 4973-4975, Jun, 1980

Base-pairing in conserved 3' end of 18 S rRNA as determined by psoralen photoreaction and RNase sensitivity

E Darzynkiewicz, K Nakashima and AJ Shatkin

Wheat and rabbit 18 rRNAs radiolabeled at the 3' end with [5'-32P]pCp were analyzed by polyacrylamide gel electrophoresis after chemical cleavage or partial enzymatic digestion under nondenaturing conditions. rRNA was similarly analyzed after photoreaction with 4'-aminomethyl- 4,5',8-trimethylpsoralen and photoreversal of the resulting pyrimidine cross-links. The digestion patterns indicated the presence of a base- paired structure within a conserved 3'-terminal sequence. This structure corresponds to the 3'-proximal hairpin configuration suggested previously for the small subunit rRNAs of Escherichia coli, yeast, rat, and silkworm on the basis of their nucleotide sequences.
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