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J. Biol. Chem., Vol. 255, Issue 11, 5126-5133, 06, 1980

Urinary oligosaccharides of mannosidosis

K Yamashita, Y Tachibana, K Mihara, S Okada, H Yabuuchi and A Kobata

Nine oligosaccharide fractions were purified from the urine of mannosidosis patients by Bio-Gel P-4 column chromatography, paper chromatography, and paper electrophoresis. Studies using exoglycosidase digestion, methylation analysis, periodate oxidation, and acetolysis revealed that most of the fractions were mixtures of two or three isomeric oligosaccharides. The structures of a total of 16 oligosaccharides were elucidated to be as follows: (formula: see text).
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