J. Biol. Chem., Vol. 255, Issue 11, 5141-5144, Jun, 1980
Complete loss of kinetoplast DNA sequences induced by ethidium bromide or by acriflavine in Trypanosoma equiperdum
GF Riou, P Belnat and J Benard
A fully kinetoplastic strain of an antigenic variant of Trypanosoma
equiperdum has been made dyskinetoplastic by successive treatments of
infected rats with ethidium bromide or acriflavine. After seven passages in
the absence of the dye, the loss of kinetoplast DNA (kDNA) is stable and
complete as shown by analytical ultracentrifugation, CsCl- dye gradients,
and the reassociation kinetics of in vitro-labeled kDNA. Moreover, these
trypanosomes exhibit the same infectivity as the wild type. These results
suggest that neither the minicircles nor the maxicircles of the kDNA
network are essential to the viability and the pathogenicity of
blood-stream trypanosomes.
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