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J. Biol. Chem., Vol. 255, Issue 12, 5514-5516, 06, 1980

Identification of platelet activating factor isolated from rabbit basophils as acetyl glyceryl ether phosphorylcholine

DJ Hanahan, CA Demopoulos, J Liehr and RN Pinckard

Platelet activating factor obtained from antigen-stimulated rabbit buffy coat leukocytes containing 10% IgE-sensitized basophils was shown by gas-liquid chromatographic and mass spectrometric analysis of products after acetolysis and by base and acid hydrolysis to be acetyl- alkylglyceryl phosphorylcholine. The alkyl side chains were composed of octadecyl (greater than 90%) and hexadecyl (less than 10%) residues.
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