HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Thomas, K. A. Articles by Bradshaw, R. A. Search for Related Content PubMed PubMed Citation Articles by Thomas, K. A. Articles by Bradshaw, R. A. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 255, Issue 12, 5517-5520, Jun, 1980

Brain fibroblast growth factor: nonidentity with myelin basic protein fragments

KA Thomas, MC Riley, SK Lemmon, NC Baglan and RA Bradshaw

Fibroblast growth factor (FGF) from bovine brain has been reported to be a family of three polypeptide fragments derived by limited proteolysis from myelin basic protein (MBP) (Westall, F. C., Lennon, V. A., and Gosopodarowicz, D. (1978) Proc. Natl. Acad. Sci. U. S. A. 75, 4675-4678). However, fragments of sequence similar to the proposed active ones, generated from bovine MBP by acid proteases, are inactive in stimulating [3H]thymidine incorporation in BALB/c 3T3 cells. Further, the principal active component of the brain FGF preparation (Gospodarowicz, D., Bialecki, H., and Greenburg, G. (1978) J. Biol. Chem. 253, 3736-3743) which can be recovered in high yield from isoelectric focusing in sucrose has a pI between 4.8 and 5.8 in contradistinction to the MBP fragments (pI approximately 10) and is not retained on a column of chicken anti-bovine MBP-Sepharose. Therefore, although the reported preparation of brain FGF gives an increase in activity units/mg of protein of about 1000-fold over the crude brain extract, the main protein components, the MBP fragments, do not possess the mitogenic activity. Additional purification of as much as 50- to 100-fold may be required to obtain a homogeneous preparation of the real brain FGF.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G Gimenez-Gallego, J Rodkey, C Bennett, M Rios-Candelore, J DiSalvo, and K Thomas Brain-derived acidic fibroblast growth factor: complete amino acid sequence and homologies Science, December 20, 1985; 230(4732): 1385 - 1388. [Abstract] [PDF]

				J. Brockes Mitogenic growth factors and nerve dependence of limb regeneration Science, September 21, 1984; 225(4668): 1280 - 1287. [Abstract] [PDF]

				T Maciag, R. Nemore, R Weinstein, and B. Gilchrest An endocrine approach to the control of epidermal growth: serum-free cultivation of human keratinocytes Science, March 27, 1981; 211(4489): 1452 - 1454. [Abstract] [PDF]