HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tso, J. Y. Articles by Zalkin, H. Search for Related Content PubMed PubMed Citation Articles by Tso, J. Y. Articles by Zalkin, H. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 255, Issue 14, 6734-6738, 07, 1980

Mechanism of inactivation of glutamine amidotransferases by the antitumor drug L-(alpha S, 5S)-alpha-amino-3-chloro-4,5-dihydro-5- isoxazoleacetic acid (AT-125)

JY Tso, SG Bower and H Zalkin

L-(alphaS, 5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid (AT-125), an antitumor drug isolated from Streptomyces sviceus, is an active site-directed affinity analog of glutamine. It selectively inactivates the glutamine-dependent activities of two bacterial glutamine amidotransferases, anthranilate synthase and glutamate synthase. A reversible noncovalent complex is formed prior to irreversible enzyme modification. Inactivation of anthranilate synthase results from incorporation of approximately 1 eq of AT-125/enzyme protomer. Active site cysteine-83 in Serratia marcescens anthranilate synthase Component II is the residue alkylated by AT-125. Anthranilate synthase is rapidly inactivated by AT-125 IN S. marcescens cells. In vivo inactivation is by the same mechanism as in vitro.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. L. Johnston, K. E. Kitson, J. W. Tweedie, S. R. Davis, and J. Lee {gamma}-Glutamyl Transpeptidase Inhibition Suppresses Milk Protein Synthesis in Isolated Ovine Mammary Cells J Dairy Sci, February 1, 2004; 87(2): 321 - 329. [Abstract] [Full Text] [PDF]

				B. W. Miles, J. B. Thoden, H. M. Holden, and F. M. Raushel Inactivation of the Amidotransferase Activity of Carbamoyl Phosphate Synthetase by the Antibiotic Acivicin J. Biol. Chem., February 1, 2002; 277(6): 4368 - 4373. [Abstract] [Full Text] [PDF]

				J. Nakamura, K. Straub, J. Wu, and L. Lou The Glutamine Hydrolysis Function of Human GMP Synthetase J. Biol. Chem., October 6, 1995; 270(40): 23450 - 23455. [Abstract] [Full Text] [PDF]