HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Feldman, L. Articles by Fasman, G. D. Search for Related Content PubMed PubMed Citation Articles by Feldman, L. Articles by Fasman, G. D. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 255, Issue 15, 7059-7062, Aug, 1980

Conformational changes in the H3 . H4 histone complex. Serological and circular dichroism studies

L Feldman, NV Beaudette, BD Stollar and GD Fasman

The H3.H4 complex has been extracted from calf thymus chromatin in either 0.05 M NaOAc, pH 5.0, or in 2.0 M KCl, 0.1 M KPO4, pH 6.7, and both forms have been shown to consist solely of histones H3 and H4 when examined on sodium dodecyl sulfate-polyacrylamide gels. Serological and circular dichroism analyses indicate the existence of structural differences between the two forms. Dilution of the high salt form of the complex into low salt produces a time-dependent conformational change which is related to reduction in the alpha helix content of the complex and which exposes antigenic sites on the complex. The existence of multiple forms of the H3.H4 complex may be related to the dynamic equilibrium of nucleosome structure in vivo.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?