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J. Biol. Chem., Vol. 255, Issue 16, 7728-7733, 08, 1980

Adenosylcobalamin-dependent ribonucleotide reductase from the blue- green alga, Anabaena sp. Purification and partial characterization

FK Gleason and TD Frick

The ribonucleotide reductase from Anabaena 7119 has been purified approximately 60- to 80-fold by conventional techniques and adsorption to the affinity medium, Matrix Gel Red A. The enzyme from Anabaena resembles the adenosylcobalamin-dependent reductase from Lactobacillus leichmannii, in that it is a small molecule (molecular weight 72,000) with no subunit structure as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Unlike its prototype, the Anabaena reductase is absolutely dependent on a divalent cation for activity, Ca2+ being the most effective. In addition, the Anabaena reductase shows a simple pattern of alloteric control by deoxyribonucleotides. CTP reduction is stimulated by dATP, GTP by dTTP, and ATP by dGTP. No reduction is observed in the absence of effectors, and none of the effectors inhibits enzyme activity. Thus, the Anabaena ribonucleotide reductase can be more easily studied by kinetic analysis than the Lactobacillus enzyme, and should provide additional information as to the mechanism of action of this enzyme in a photosynthetic organism.
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