HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Koduri, R. K. Articles by Gots, J. S. Search for Related Content PubMed PubMed Citation Articles by Koduri, R. K. Articles by Gots, J. S. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 255, Issue 20, 9594-9598, Oct, 1980

A DNA-binding protein with specificity for pur genes in Escherichia coli

RK Koduri and JS Gots

A DNA-binding protein was isolated from Escherichia coli using a procedure designed for selective enrichment of regulatory proteins. In this procedure, a multicopy bacterial plasmid carrying a purE operon was used to sequester and mobilize the putative regulatory protein for pur genes. Purification of the protein from a plasmid-enriched lysate was obtained by precipitation with polyethylene glycol, elution from the precipitate with high salt and fractionation by phosphocellulose, and AMP affinity chromatography. Analysis of DNA binding by nitrocellulose filter assays showed that binding of the protein required the presence of pur genes and was either ATP dependent for some genes (purE, purA) or GTP dependent for others (purF, purI). Plasmid DNA carrying the guaAB operon did not bind the protein.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?