| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 255, Issue 20, 9635-9640, Oct, 1980
JD Johnson, JH Collins, SP Robertson and JD Potter
Cardiac troponin C (C-TnC) was labeled with the sulfhydryl-specific
fluorescent probe molecule 2-(4'-iodoacetamidoanilino)naphthalene-6-
sulfonic acid at cysteine 35 and 84 to produce C-TnCIA. This modified
protein binds Ca2+, undergoes Ca2+-induced increases in alpha helix, and
forms a complex with other troponin subunits as does unlabeled C- TnC.
C-TnCIA undergoes a small fluorescence decrease with Ca2+ or Mg2+ binding
to the two high affinity Ca2+-Mg2+ sites of C-TnC and a large biphasic
approximately 2.1-fold fluorescence increase with Ca2+ binding to two lower
affinity Ca2+-specific sites with KCa of approximately 4.5 X 10(5) M-1 and
approximately 5 X 10(2) M-1. C-TnCIA was formed in a complex with troponin
I (TnI) and troponin T to form C-TnIA. This fluorescent reconstituted whole
troponin undergoes a 25% decrease with Ca2+ binding to a Ca2+-specific site
of KCa approximately 3 X 10(6) M- 1. C-TnC, therefore, contains a single
Ca2+-specific site of approximate equal affinity as the two Ca2+-specific
regulatory sites of skeletal TnC. This Ca2+-specific site in C-TnC (like
its two corresponding sites in S-TnC) undergoes an approximate 10-fold
increase in affinity in whole troponin or when TnC is complexed with TnI.
Since the two Ca2+-specific sites in skeletal troponin have been shown to
be the regulatory sites of skeletal muscle contraction we suggest that this
single Ca2+-specific site, of equal affinity, in C-TnC is the regulatory
site of cardiac muscle contraction.
A fluorescent probe study of Ca2+ binding to the Ca2+-specific sites of cardiac troponin and troponin C
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Wen, J. R. Pinto, A. V. Gomes, Y. Xu, Y. Wang, Y. Wang, J. D. Potter, and W. G. L. Kerrick Functional Consequences of the Human Cardiac Troponin I Hypertrophic Cardiomyopathy Mutation R145G in Transgenic Mice J. Biol. Chem., July 18, 2008; 283(29): 20484 - 20494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Lim, H. Yang, M. Yang, C.-K. Wang, J. Shi, E. A. Berg, D. R. Pimentel, J. K. Gwathmey, R. J. Hajjar, M. Helmes, et al. A Novel Mutant Cardiac Troponin C Disrupts Molecular Motions Critical for Calcium Binding Affinity and Cardiomyocyte Contractility Biophys. J., May 1, 2008; 94(9): 3577 - 3589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Davis and S. B. Tikunova Ca2+ exchange with troponin C and cardiac muscle dynamics Cardiovasc Res, March 1, 2008; 77(4): 619 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Solzin, B. Iorga, E. Sierakowski, D. P. Gomez Alcazar, D. F. Ruess, T. Kubacki, S. Zittrich, N. Blaudeck, G. Pfitzer, and R. Stehle Kinetic Mechanism of the Ca2+-Dependent Switch-On and Switch-Off of Cardiac Troponin in Myofibrils Biophys. J., December 1, 2007; 93(11): 3917 - 3931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Davis, C. Norman, T. Kobayashi, R. J. Solaro, D. R. Swartz, and S. B. Tikunova Effects of Thin and Thick Filament Proteins on Calcium Binding and Exchange with Cardiac Troponin C Biophys. J., May 1, 2007; 92(9): 3195 - 3206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Breukelmann and P. R. Housmans Halothane, Isoflurane, and Sevoflurane Increase the Kinetics of Ca2+-Induced Conformational Change of Recombinant Human Cardiac Troponin C Anesth. Analg., February 1, 2007; 104(2): 332 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Stelzer, D. P. Fitzsimons, and R. L. Moss Ablation of Myosin-Binding Protein-C Accelerates Force Development in Mouse Myocardium Biophys. J., June 1, 2006; 90(11): 4119 - 4127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kobayashi and R. J. Solaro Increased Ca2+ Affinity of Cardiac Thin Filaments Reconstituted with Cardiomyopathy-related Mutant Cardiac Troponin I J. Biol. Chem., May 12, 2006; 281(19): 13471 - 13477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Umeki, T. Mitsui, K. Kondo, and S. Maruta Conformational change of the loop L5 in rice Kinesin motor domain induced by nucleotide binding. J. Biochem., May 1, 2006; 139(5): 857 - 864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Niederer, P. J. Hunter, and N. P. Smith A Quantitative Analysis of Cardiac Myocyte Relaxation: A Simulation Study Biophys. J., March 1, 2006; 90(5): 1697 - 1722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Gong, V. Hatch, L. Ali, W. Lehman, R. Craig, and L. S. Tobacman Mini-thin filaments regulated by troponin-tropomyosin PNAS, January 18, 2005; 102(3): 656 - 661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Gomes, G. Venkatraman, J. P. Davis, S. B. Tikunova, P. Engel, R. J. Solaro, and J. D. Potter Cardiac Troponin T Isoforms Affect the Ca2+ Sensitivity of Force Development in the Presence of Slow Skeletal Troponin I: INSIGHTS INTO THE ROLE OF TROPONIN T ISOFORMS IN THE FETAL HEART J. Biol. Chem., November 26, 2004; 279(48): 49579 - 49587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Regnier, H. Martin, R. J. Barsotti, A. J. Rivera, D. A. Martyn, and E. Clemmens Cross-Bridge versus Thin Filament Contributions to the Level and Rate of Force Development in Cardiac Muscle Biophys. J., September 1, 2004; 87(3): 1815 - 1824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Tikunova and J. P. Davis Designing Calcium-sensitizing Mutations in the Regulatory Domain of Cardiac Troponin C J. Biol. Chem., August 20, 2004; 279(34): 35341 - 35352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Tobacman, D. Lin, C. Butters, C. Landis, N. Back, D. Pavlov, and E. Homsher Functional Consequences of Troponin T Mutations Found in Hypertrophic Cardiomyopathy J. Biol. Chem., October 1, 1999; 274(40): 28363 - 28370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. L. Korman and L. S. Tobacman Mutations in Actin Subdomain 3 That Impair Thin Filament Regulation by Troponin and Tropomyosin J. Biol. Chem., August 6, 1999; 274(32): 22191 - 22196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M. Rarick, X.-H. Tu, R. J. Solaro, and A. F. Martin The C Terminus of Cardiac Troponin I Is Essential for Full Inhibitory Activity and Ca2+ Sensitivity of Rat Myofibrils J. Biol. Chem., October 24, 1997; 272(43): 26887 - 26892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M. Rarick, T. J. Opgenorth, T. W. von Geldern, J. R. Wu-Wong, and R. J. Solaro An Essential Myosin Light Chain Peptide Induces Supramaximal Stimulation of Cardiac Myofibrillar ATPase Activity J. Biol. Chem., October 25, 1996; 271(43): 27039 - 27043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-j. Dong, S. S. Rosenfeld, C.-K. Wang, A. M. Gordon, and H. C. Cheung Kinetic Studies of Calcium Binding to the Regulatory Site of Troponin C from Cardiac Muscle J. Biol. Chem., January 12, 1996; 271(2): 688 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Pearlstone, M. Chandra, M. M. Sorenson, and L. B. Smillie Biological Function and Site II Ca2+-induced Opening of the Regulatory Domain of Skeletal Troponin C Are Impaired by Invariant Site I or II Glu Mutations J. Biol. Chem., November 3, 2000; 275(45): 35106 - 35115. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
