JBC Ideal method for primary cell transfection

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J. Biol. Chem., Vol. 255, Issue 22, 10563-10565, Nov, 1980

Mapping of mitochondrial structural genes in Neurospora crassa

G Macino

A hybridization method has been employed to study the organization of the mitochondrial genome of Neurospora crassa. The method involves the use of 5' end-labeled single-stranded restriction fragments obtained from cytoplasmic "petite" strains of Saccharomyces cerevisiae known to contain single mitochondrial genes. The presence and localization of genes homologous to Subunits 1, 2, and 3 of cytochrome oxidase, cytochrome b and Subunit 6 of the ATPase is thus established for the mitochondrial genome of N. crassa.
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