J. Biol. Chem., Vol. 255, Issue 23, 11284-11289, 12, 1980
Subunit interactions inhibit the binding of beta nerve growth factor to receptors on embryonic chick sensory neurons
RM Harris-Warrick, MA Bothwell and EM Shooter
The binding of the beta nerve growth factor subunit in the 7 S nerve growth
factor complex to the two nerve growth factor receptors on chick embryo
dorsal root ganglion cells was investigated. Under conditions where the 7 S
nerve growth factor complex is maximally stable (in the presence of excess
alpha and gamma subunits and of 20 to 30 microM zinc ion), no binding to
either receptor was detectable. The time course of the decrease in the
binding of beta nerve growth factor to the receptors upon addition of alpha
and gamma subunits and zinc ion paralleled the formation of the 7 S
complex. Addition of alpha and gamma subunits and zinc ion to the
bisdes-arginine118-nerve growth factor, which does not re-form the 7 S
complex, failed to inhibit the binding of the derivative to either
receptor. While the alpha subunit alone had no effect on beta nerve growth
factor binding, the gamma subunit decreased its binding in proportion to
the amount of complex formed between these two subunits, suggesting that
the beta . gamma complex, like the 7 S complex, does not bind to nerve
growth factor receptors.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
