HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Levy, G. N. Articles by Aminoff, D. Search for Related Content PubMed PubMed Citation Articles by Levy, G. N. Articles by Aminoff, D. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 255, Issue 24, 11737-11742, 12, 1980

Purification and properties of alpha-N-acetylgalactosaminidase from Clostridium perfringens

GN Levy and D Aminoff

Exo-alpha-N-acetylgalactosaminidase has been purified 8000-fold from Clostridium perfringens by gel filtration, ion exchange chromatography, isoelectric precipitation, and negative adsorption on human O type erythrocytes. The resulting enzyme is active at physiological pH and temperature. Phenyl glycosides, oligosaccharides, mucins, glycolipids, and cell membranes are substrates for this enzyme. The result of enzyme action on blood type A erythrocytes is the loss of A activity and the simultaneous appearance of H activity, characteristic of the O blood group type. Polyacrylamide gel electrophoresis in sodium dodecyl sulfate demonstrates electrophoresis in sodium dodecyl sulfate demonstrates that the blood group A-destroying activity is distinct from the other glycosidase activities found in C. perfringens.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Zuniga, I. Comas, R. Linaje, V. Monedero, M. J. Yebra, C. D. Esteban, J. Deutscher, G. Perez-Martinez, and F. Gonzalez-Candelas Horizontal Gene Transfer in the Molecular Evolution of Mannose PTS Transporters Mol. Biol. Evol., August 1, 2005; 22(8): 1673 - 1685. [Abstract] [Full Text] [PDF]

				L. C. Hoskins, E. T. Boulding, and G. Larson Purification and Characterization of Blood Group A-degrading Isoforms of alpha -N-Acetylgalactosaminidase from Ruminococcus torques Strain IX-70 J. Biol. Chem., March 21, 1997; 272(12): 7932 - 7939. [Abstract] [Full Text] [PDF]