HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bonitz, S. G. Articles by Macino, G. Search for Related Content PubMed PubMed Citation Articles by Bonitz, S. G. Articles by Macino, G. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 255, Issue 24, 11922-11926, 12, 1980

Assembly of the mitochondrial membrane system. Physical map of the Oxi3 locus of yeast mitochondrial DNA

SG Bonitz, G Coruzzi, BE Thalenfeld, A Tzagoloff and G Macino

The oxi3 locus of yeast mitochondrial DNA is currently thought to code for Subunit 1 of cytochrome oxidase (Tzagoloff, A., Macino, G., and Sebald, W. (1979) Annu. Rev. Biochem. 48, 419-441). The respiratory competent strain of Saccharomyces cerevisiae D273-10B/A48 was used to obtain cytoplasmic "petite" clones enriched for genetic markers in the oci3 locus. The most complex clone studied (DS6) was ascertained to have a mitochondrial genome with a tandemly repeated segment of mtDNA 16.5 kilobases in length. The oxi3 locus was dissected by mutagenesis of DS6 with ethidium bromide and selection of new clones having less complex genotypes. Six derivative clones with genome sizes ranging from 2.3 to 6.1 kilobases have been extensively analyzed. Most of the restriction sites present in the segments of mtDNA retained by the clones have been mapped, thereby providing a detailed restriction map of the oxi3 gene. Based on the physical locations of the most distal oxi3 mutations, the gene spans approximately 10,000 nucleotides and occupies the region of wild type mtDNA from 44 to 58 map units.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				W. Chen, M. A. Islas-Osuna, and C. L. Dieckmann Suppressor Analysis of Mutations in the 5'-Untranslated Region of COB mRNA Identifies Components of General Pathways for Mitochondrial mRNA Processing and Decay in Saccharomyces cerevisiae Genetics, April 1, 1999; 151(4): 1315 - 1325. [Abstract] [Full Text]

				C. Dagsgaard, L. E. Taylor, K. M. O'Brien, and R. O. Poyton Effects of Anoxia and the Mitochondrion on Expression of Aerobic Nuclear COX Genes in Yeast. EVIDENCE FOR A SIGNALING PATHWAY FROM THE MITOCHONDRIAL GENOME TO THE NUCLEUS J. Biol. Chem., March 2, 2001; 276(10): 7593 - 7601. [Abstract] [Full Text] [PDF]