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J. Biol. Chem., Vol. 255, Issue 8, 3263-3265, Apr, 1980
JL Garwin, AL Klages and JE Cronan Jr
Cvc- mutants of Escherichia coli are deficient in the synthesis of cis- vaccenic acid and in the temperature control of fatty acid synthesis. In this communication, it is demonstrated that these mutants lack beta- ketoacyl-acyl carrier protein synthase II. The deficiencies in cis- vaccenate synthesis and synthase II are shown to be due to a lesion in the same gene, fabF. Lesions in the fabF gene are found to affect growth only when the strain also carries a lesion in the fabB gene, the structural gene for beta-ketoacyl-acyl carrier protein synthase I.
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