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J. Biol. Chem., Vol. 256, Issue 11, 5422-5427, 06, 1981
MA Shotwell, DW Jayme, MS Kilberg and DL Oxender
The neutral amino acid transport systems A, ASC, and L have been
characterized in the Chinese hamster ovary cell. System A, defined by its
sodium ion dependency and inhibition by 2-methylaminoisobutyric acid, was
found to be extremely sensitive to the pH of the external medium and to
increase in response to starvation for amino acids. System ASC, identified
by its sodium ion dependency and intolerance of N-methylation of
substrates, was found to be relatively insensitive to external pH and
nutrient limitation. System ASC in Chinese hamster ovary cells has been
shown to be the major mode of entry of neutral amino acids. A much broader
substrate specificity was observed for System ASC than has been reported
for other mammalian cell types, with nearly every amino acid tested showing
significant uptake by this system. In addition, the highest observed
velocities of uptake were for System ASC. System L, defined by its sodium
ion independency and inhibition by
2-aminobicyclo-[2,2,2]-heptane-2-carboxylic acid, was enhanced in activity
by lowered pH. The starvation-induced enhancement in System A activity was
prevented by the presence of a single substrate of this system, the
nonmetabolizable analog 2- methylaminoisobutyric, or by the presence of the
protein synthesis inhibitor cycloheximide.
Neutral amino acid transport systems in Chinese hamster ovary cells
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