J. Biol. Chem., Vol. 256, Issue 2, 701-706, Jan, 1981
Glycosylation causes an apparent block in translation of immunoglobulin heavy chain
LW Bergman, E Harris and WM Kuehl
Analysis of nascent heavy chains isolated from MPC11 (gamma 2b heavy
chains) and MOPC 21 (gamma 1 heavy chains) mouse myeloma cells demonstrates
an accumulation of nascent heavy chains which are slightly smaller in mass
(approximately 35,000 daltons) than nascent heavy chains which have just
been glycosylated (approximately 38,000 daltons). The accumulation of
35,000-dalton nascent heavy chain appears to be a consequence of the
glycosylation process since tunicamycin, an inhibitor of glycosylation,
abolishes the apparent translational block manifested by the accumulation
of 35,000-dalton nascent chains. Tunicamycin also causes a 15 to 25%
increase n the relative rate of synthesis of heavy chain compared to the
corresponding rate of synthesis of the nonglycosylated light chain
synthesized by the same cell. These results suggest that the translation
block, caused by the glycosylation process, of heavy chain synthesis
contributes to the imbalance of heavy chain and light chain biosynthesis
observed in malignant and normal lymphoid cells.
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