HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Shaklai, N. Articles by Morgan, W. T. Search for Related Content PubMed PubMed Citation Articles by Shaklai, N. Articles by Morgan, W. T. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 256, Issue 4, 1544-1548, 02, 1981

The interaction of heme-hemopexin with CO

N Shaklai, VS Sharma, U Muller-Eberhard and WT Morgan

The equilibria and kinetics of the reaction of heme-hemopexin with CO were studied. A stoichiometry of one CO/heme was determined, and the affinity of heme-hemopexin for CO was found to be pH-dependent. At pH 8.0, the affinity constant was 4.5 X 10(5) M-1 compared with 4 X 10(6) M-1 at pH 6.1. The kinetics of CO binding were also pH-dependent. A biphasic reaction at neutral pH could be resolved into a faster phase (kon = 2.2 X 10(3) M-1 s-1) solely found at pH 6.0, and a slower phase (kon = 2.0 X 10(2) M-1 s-1) solely found at pH 8.0. The dissociation reaction on the other hand was found to be independent of pH in the range examined (koff = 5 X 10(-4) s-1).
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?