HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, C. L. Articles by Gergely, J. Search for Related Content PubMed PubMed Citation Articles by Wang, C. L. Articles by Gergely, J. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 258, Issue 15, 9175-9177, Aug, 1983

Kinetics of Ca2+ release shows interactions between the two classes of sites of troponin-C

CL Wang, PC Leavis and J Gergely

The kinetics of Ca2+-release from the two high affinity sites of troponin-C (TnC) was studied by the stopped flow technique following rapid mixing with either EDTA or excess TbCl3. The rate constants obtained by the two methods were 2.8 and 0.7 s-1, respectively. For the tryptic fragment of TnC that contains only the COOH-terminal half of the molecule, both methods generate rate constants of 2.2 s-1. These results are consistent with the interpretation that binding of Tb3+ to the Ca2+-specific sites reduces the rate of dissociation of Ca2+ from, and thereby enhances the affinity for, the Ca2+-Mg2+ sites; this, in turn, suggests interactions between the two halves of the TnC molecule.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J.-M. Francois, Z. Sheng, D. Szczesna, and J. D. Potter The Functional Role of the Domains of Troponin-C Investigated with Thrombin Fragments of Troponin-C Reconstituted into Skinned Muscle Fibers J. Biol. Chem., August 18, 1995; 270(33): 19287 - 19293. [Abstract] [Full Text] [PDF]