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J. Biol. Chem., Vol. 258, Issue 15, 9192-9196, 08, 1983

Purification of the bacteriophage lambda late gene regulator encoded by gene Q

EJ Grayhack and JW Roberts

The product of bacteriophage lambda gene Q is a transcription antiterminator that activates phage late gene expression. We report a method to purify the lambda Q protein to near homogeneity. We have followed during purification both activity of Q protein to provoke in vitro synthesis of the lambda late protein endolysin in the DNA- dependent protein-synthesizing system, and radioactivity in a polypeptide that we show by genetic criteria to be lambda Q. These co- purify throughout the procedure.
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