HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Baldassare, J. J. Search for Related Content PubMed PubMed Citation Articles by Baldassare, J. J. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 258, Issue 17, 10223-10226, Sep, 1983

Enhancement of the reconstituted glucose transport activity from LM cells by phosphatidylethanolamine

JJ Baldassare

The glucose transport activity from LM cells was solubilized with sodium cholate and reconstituted into liposomes containing phospholipids of varied polar head group composition. The reconstituted vesicles exhibited time-dependent preferential uptake of D- versus L- glucose. Phloretin and mercuric chloride, known inhibitors of glucose transport in the intact cells, inhibited the reconstituted transport activity. The transport activity was found to be sensitive to the phospholipid composition of the reconstituted vesicles. Proteoliposomes containing phosphatidylethanolamine showed increased transport activity. In addition, incubation of reconstituted vesicles containing phosphatidylcholine with phospholipase D plus ethanolamine resulted in vesicles containing phosphatidylcholine plus phosphatidylethanolamine and increased transport activity. These results indicate that the glucose transport system of LM cells is sensitive to polar head group structure of the phospholipids.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?