HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tate, W. P. Articles by Luhrmann, R. Search for Related Content PubMed PubMed Citation Articles by Tate, W. P. Articles by Luhrmann, R. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 258, Issue 17, 10360-10365, Sep, 1983

Recognition of termination codon by release factor in the presence of a tRNA-occupied A site. Evidence for flexibility in accommodation of the release factor on the ribosome

WP Tate, H Hornig and R Luhrmann

Recognition of the termination codon by release factor was studied with 70 S ribosomes containing initiator tRNA at the P site and an amino acid-specifying codon as well as the corresponding cognate tRNA at the A site. In vitro termination was not excluded when either the A site was occupied with an amino acid-specifying codon or the termination codon was displaced from the A site codon position by spacer nucleotides between UAA and the P site bound AUG. The release factor may have flexibility in its codon recognizing domain to be able to adjust to this enforced change in the position of the termination codon on the ribosome without loss of functional specificity. Deacylated tRNA bound at the A site of 70 S ribosomes did not interfere with stoichiometric UAA-directed release factor binding to these particles. In contrast, a ternary complex (aminoacyl-tRNA elongation factor-Tu . GTP) abolished the binding of the release factor while aminoacyl-tRNA inhibited it only weakly. This suggests that release factor and elongation factor-Tu have overlapping binding sites but the A site binding domains of the release factor and tRNA are exclusive.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				E. Miyamoto-Sato, N. Nemoto, K. Kobayashi, and H. Yanagawa Specific bonding of puromycin to full-length protein at the C-terminus Nucleic Acids Res., March 1, 2000; 28(5): 1176 - 1182. [Abstract] [Full Text] [PDF]

				G. Grentzmann and P. J. Kelly Ribosomal Binding Site of Release Factors RF1 and RF2. A NEW TRANSLATIONAL TERMINATION ASSAY IN VITRO J. Biol. Chem., May 9, 1997; 272(19): 12300 - 12304. [Abstract] [Full Text] [PDF]