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J. Biol. Chem., Vol. 259, Issue 11, 7137-7142, Jun, 1984
MA Beaven, JP Moore, GA Smith, TR Hesketh and JC Metcalfe
Phosphatidylinositol (PI) and its phosphorylated derivatives are rapidly
broken down in 2H3 cells stimulated with antigen, with a time course which
coincides with the generation of the Ca signal. Stimulated PI breakdown is
absolutely dependent on Ca2+ in the medium with a concentration dependence
similar to that of the Ca signal and histamine release described in the
preceding paper. However, PI breakdown does not depend on the rise in free
cytoplasmic Ca2+ concentration in stimulated cells over the range 100 nM to
1 microM. Thus, stimulation by the ionophore A23187 causes only a small
increase in PI breakdown and the Ca signal stimulated by antigen can be
selectively blocked with appropriate concentrations of Zn2+ (100 microM) or
La3+ (10-100 microM) which have small or negligible effects on stimulated
PI breakdown. Both PI breakdown and the Ca signal appear to depend on a
common external Ca2+ site (or sites) with Km approximately equal to 0.4 mM,
and the data are consistent with either independent activation of PI
phosphodiesterase and the Ca signal after antigenic stimulation, or with PI
breakdown as a component of the mechanism by which the Ca signal is
generated.
The calcium signal and phosphatidylinositol breakdown in 2H3 cells
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