| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 259, Issue 15, 9580-9586, Aug, 1984
DJ Brown and JA Gordon
We have observed that intact wild type Rous sarcoma virus-transformed chicken embryo fibroblasts (CEF), when incubated with micromolar sodium orthovanadate for as little as 4 h, results in a 2-3-fold increase in pp60v-src kinase activity as judged by the IgG kinase assay. The addition of sodium vanadate or vanadyl sulfate to transformed cell lysates or the immunoprecipitate assay was without effect on the activity of isolated pp60v-src kinase activity. The increased kinase activity is reflected intracellularly by an increased phosphotyrosine content of a known substrate of pp60v-src, a 36-kDa phosphoprotein, without a change in total phosphate incorporation into cellular proteins. Pulse and pulse-chase experiments with [35S]methionine indicated that there was no change in the net or rate of biosynthesis of pp60v-src which might have accounted for the elevated kinase activity. Along with this stimulation of pp60v-src kinase activity, we found a parallel increase in the phosphate content of the enzyme and the appearance of an electrophoretic variant after vanadate treatment. The increased phosphorylation of the kinase is accounted for by an increase in phosphotyrosine content without a change in phosphoserine content. The increased phosphotyrosine content is shown to be resident in the carboxyl-terminal fragment and, unexpectedly, in the amino- terminal fragment of the kinase. The phosphotyrosine present in the amino-terminal portion may be a de novo phosphorylation, but could also represent a tyrosine site(s) not easily detected under unstimulated conditions in the absence of vanadate.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. A. Schumacher, D. K. Crockett, K. S.J. Elenitoba-Johnson, and M. S. Lim Evaluation of Enrichment Techniques for Mass Spectrometry: Identification of Tyrosine Phosphoproteins in Cancer Cells J. Mol. Diagn., April 1, 2007; 9(2): 169 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Tagawa, A. Mezzacasa, A. Hayer, A. Longatti, L. Pelkmans, and A. Helenius Assembly and trafficking of caveolar domains in the cell: caveolae as stable, cargo-triggered, vesicular transporters J. Cell Biol., August 29, 2005; 170(5): 769 - 779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Satinover, C. A. Leach, P. T. Stukenberg, and D. L. Brautigan Activation of Aurora-A kinase by protein phosphatase inhibitor-2, a bifunctional signaling protein PNAS, June 8, 2004; 101(23): 8625 - 8630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mukherjee, L. Arnaud, and J. A. Cooper Lipid-dependent Recruitment of Neuronal Src to Lipid Rafts in the Brain J. Biol. Chem., October 17, 2003; 278(42): 40806 - 40814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Min, E.-G. Kim, and J. H. Exton Involvement of Tyrosine Phosphorylation and Protein Kinase C in the Activation of Phospholipase D by H2O2 in Swiss 3T3 Fibroblasts J. Biol. Chem., November 6, 1998; 273(45): 29986 - 29994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. V. Smirnov and P. I. Aaronson Inhibition of Vascular Smooth Muscle Cell K+ Currents by Tyrosine Kinase Inhibitors Genistein and ST 638 Circ. Res., February 1, 1995; 76(2): 310 - 316. [Abstract] [Full Text]
|
|
|
|
 |
|
 F Monier-Gavelle and J. Duband Control of N-cadherin-mediated intercellular adhesion in migrating neural crest cells in vitro J. Cell Sci., January 12, 1995; 108(12): 3839 - 3853. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Cooper, K. Gould, C. Cartwright, and T Hunter Tyr527 is phosphorylated in pp60c-src: implications for regulation Science, March 21, 1986; 231(4744): 1431 - 1434. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
