HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Georgoff, I. Articles by Isom, H. C. Search for Related Content PubMed PubMed Citation Articles by Georgoff, I. Articles by Isom, H. C. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 259, Issue 15, 9595-9602, 08, 1984

Effect of simian virus 40 infection on albumin production by hepatocytes cultured in chemically defined medium and plated on collagen and non-collagen attachment surfaces

I Georgoff, T Secott and HC Isom

Simian virus 40 (SV40)-infected rat hepatocytes were tested for albumin production (a marker of differentiated hepatocyte function) by growth in serum-free medium for 30-40 days. We observed a biphasic peak of albumin production, with the first peak at 4-6 and the second at 10-20 days after plating. A 2- to 3-fold increase in the second peak was observed when albumin production by SV40-infected cultures was compared with that by uninfected cultures. No increase in cell number in SV40- infected cultures was observed during this 20-day period. By 25-35 days, albumin production by uninfected cultures ceased but was detected in 50-60% of SV40-infected cultures. SV40-induced stimulation and persistence occurred regardless of the attachment surface and did not require collagen. Transformation occurred regardless of whether stimulation or persistence of albumin production was seen; however, stimulation and persistence were necessary to yield transformed cultures that produced albumin.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?