| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 259, Issue 16, 9983-9986, Aug, 1984
MF Carlier, D Pantaloni and ED Korn
The correlation between the time courses of actin polymerization under continuous sonication and the associated ATP hydrolysis has been studied. ATP hydrolysis was not mechanistically coupled to polymerization, i.e. not necessary for polymerization, but occurred on F-actin in a subsequent monomolecular reaction. Under sonication, polymerization was complete in 10 s while hydrolysis of ATP on the polymer required 200 s. A value of 0.023 s-1 was found for the first order rate constant of ATP hydrolysis on the polymer at 25 degrees C, pH 7.8, in the presence of 0.2 mM ATP, 0.1 mM CaCl2, and 1 mM MgCl2, independent of the F-actin concentration. The conversion of ATP X F- actin to ADP X F-actin was accompanied by an increase in fluorescence of a pyrenyl probe covalently attached to actin, consistent with a 2- fold greater fluorescence for ADP X F-actin than for ATP X F-actin, with a rate constant of 0.022 s-1. In contrast, the fluorescence of F- actin labeled with 7-chloro-4-nitrobenzeno-2-oxa-1,3-diazole did not change significantly when ATP or ADP was bound. The direct consequence of the uncoupling between polymerization and ATP hydrolysis is the formation of an ATP cap at the ends of the filaments, which maintains the stability of the polymer, while most of the filament contains bound ADP. The heterogeneity of the filament with respect to ATP and ADP results in a nonlinear relationship between the rate of elongation and the concentration of G-actin with a discontinuity at the critical concentration, where the rate of growth is zero. In this respect, F- actin in ATP behaves similarly to microtubules in GTP.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  F. J. Brooks and A. E. Carlsson Actin Polymerization Overshoots and ATP Hydrolysis as Assayed by Pyrene Fluorescence Biophys. J., August 1, 2008; 95(3): 1050 - 1062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Iwasa, K. Maeda, A. Narita, Y. Maeda, and T. Oda Dual Roles of Gln137 of Actin Revealed by Recombinant Human Cardiac Muscle {alpha}-Actin Mutants J. Biol. Chem., July 25, 2008; 283(30): 21045 - 21053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Liu, S. Shu, M.-S. S. Hong, R. L. Levine, and E. D. Korn Phosphorylation of actin Tyr-53 inhibits filament nucleation and elongation and destabilizes filaments PNAS, September 12, 2006; 103(37): 13694 - 13699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Vavylonis, Q. Yang, and B. O'Shaughnessy Actin polymerization kinetics, cap structure, and fluctuations PNAS, June 14, 2005; 102(24): 8543 - 8548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. D. Korn The Discovery of Unconventional Myosins: Serendipity or Luck? J. Biol. Chem., March 5, 2004; 279(10): 8517 - 8525. [Full Text] [PDF]
|
|
|
|
 |
|
 V. E. Galkin, A. Orlova, M. S. VanLoock, A. Shvetsov, E. Reisler, and E. H. Egelman ADF/cofilin use an intrinsic mode of F-actin instability to disrupt actin filaments J. Cell Biol., December 8, 2003; 163(5): 1057 - 1066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Dos Remedios, D. Chhabra, M. Kekic, I. V. Dedova, M. Tsubakihara, D. A. Berry, and N. J. Nosworthy Actin Binding Proteins: Regulation of Cytoskeletal Microfilaments Physiol Rev, April 1, 2003; 83(2): 433 - 473. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Suetsugu, H. Miki, H. Yamaguchi, T. Obinata, and T. Takenawa Enhancement of branching efficiency by the actin filament-binding activity of N-WASP/WAVE2 J. Cell Sci., March 14, 2002; 114(24): 4533 - 4542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Gutsche-Perelroizen, J. Lepault, A. Ott, and M.-F. Carlier Filament Assembly from Profilin-Actin J. Biol. Chem., March 5, 1999; 274(10): 6234 - 6243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Didry, M.-F. Carlier, and D. Pantaloni Synergy between Actin Depolymerizing Factor/Cofilin and Profilin in Increasing Actin Filament Turnover J. Biol. Chem., October 2, 1998; 273(40): 25602 - 25611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Ressad, D. Didry, G.-X. Xia, Y. Hong, N.-H. Chua, D. Pantaloni, and M.-F. Carlier Kinetic Analysis of the Interaction of Actin-depolymerizing Factor (ADF)/Cofilin with G- and F-Actins. COMPARISON OF PLANT AND HUMAN ADFs AND EFFECT OF PHOSPHORYLATION J. Biol. Chem., August 14, 1998; 273(33): 20894 - 20902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Perelroizen, D. Didry, H. Christensen, N.-H. Chua, and M.-F. Carlier Role of Nucleotide Exchange and Hydrolysis in the Function of Profilin in Actin Assembly J. Biol. Chem., May 24, 1996; 271(21): 12302 - 12309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-F. Carlier, D. Didry, I. Erk, J. Lepault, M. L. Van Troys, J.ėl Vandekerckhove, I. Perelroizen, H. Yin, Y. Doi, and D. Pantaloni Tbeta(4) Is Not a Simple G-actin Sequestering Protein and Interacts with F-actin at High Concentration J. Biol. Chem., April 19, 1996; 271(16): 9231 - 9239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Perelroizen, M.-F. Carlier, and D. Pantaloni Binding of Divalent Cation and Nucleotide to G-actin in the Presence of Profilin J. Biol. Chem., January 27, 1995; 270(4): 1501 - 1508. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Korn, M. Carlier, and D Pantaloni Actin polymerization and ATP hydrolysis Science, October 30, 1987; 238(4827): 638 - 644. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
