JBC Ideal method for primary cell transfection

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J. Biol. Chem., Vol. 259, Issue 17, 10645-10648, 09, 1984

A v-sis oncogene protein produced in bacteria competes for platelet- derived growth factor binding to its receptor

JY Wang and LT Williams

The oncogene of simian sarcoma virus, v-sis, encodes a protein which is homologous to human platelet-derived growth factor (PDGF). This v-sis- encoded protein was expressed in bacteria using an inducible promotor of lambda phage. Soluble extracts from these bacteria contained a substance which competed with 125I-PDGF for PDGF receptor sites in fibroblast membranes. The receptor competition activity was correlated with the presence of the v-sis-encoded protein as assessed by genetic and immunochemical criteria. These results directly demonstrate that the v-sis oncogene product is functionally related to PDGF.
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