J. Biol. Chem., Vol. 259, Issue 17, 10733-10737, Sep, 1984

Insect apolipophorin III. Purification and properties

JK Kawooya, PS Keim, RO Ryan, JP Shapiro, P Samaraweera and JH Law

The hemolymph of adult Manduca sexta (tobacco hornworm) contains a 17,000-dalton protein that can associate reversibly with the insect lipoprotein lipophorin. The protein is abundant in the hemolymph of the adult, but is found in larval hemolymph in only small amounts, and does not associate with larval lipophorin. On the basis of its association with adult lipophorin, we have designated the protein apolipophorin III. Apolipophorin III was dissociated from adult lipophorin by guanidinium chloride treatment and isolated by gel permeation and ion exchange chromatography. The unassociated apolipophorin III was also purified from lipophorin-free hemolymph by gel permeation, ion exchange, and lectin chromatography. Both preparations have identical isoelectric points and amino acid composition as well as the following properties. Apolipophorin III is a non-glycosylated polypeptide lacking cysteine and tryptophan. The 17,000-dalton polypeptide dimerizes in solution to a protein of Mr = 34,000.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. S. Kiss, P. M. M. Weers, V. Narayanaswami, J. Cohen, C. M. Kay, and R. O. Ryan Structure-guided Protein Engineering Modulates Helix Bundle Exchangeable Apolipoprotein Properties J. Biol. Chem., June 6, 2003; 278(24): 21952 - 21959. [Abstract] [Full Text] [PDF]

				J. Wang, S. M. Gagne, B. D. Sykes, and R. O. Ryan Insight into Lipid Surface Recognition and Reversible Conformational Adaptations of an Exchangeable Apolipoprotein by Multidimensional Heteronuclear NMR Techniques J. Biol. Chem., July 18, 1997; 272(29): 17912 - 17920. [Abstract] [Full Text] [PDF]