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J. Biol. Chem., Vol. 259, Issue 2, 1127-1131, 01, 1984
DR Tolan, AB Amsden, SD Putney, MS Urdea and EE Penhoet
The complete nucleotide sequence of rabbit muscle aldolase mRNA has been
determined from recombinant cDNA clones and from a primer-extended cDNA
synthesis on the mRNA template. The sequence is composed of 1375
nucleotides, exclusive of the poly(A) tails. The 5'-untranslated region
contains 62 bases including a potential ribosome-binding site. The 3'-
untranslated region is 221 bases long. The remaining 1092 nucleotides are
in an open reading frame coding for 364 amino acids. There is a single
methionine residue preceding the NH2-terminal proline. The deduced amino
acid sequence corrects a number of discrepancies between published
structures as well as assignments previously missed. The sequences of the
cloned cDNAs suggests there may be microheterogeneity in the messenger RNA
population.
The complete nucleotide sequence for rabbit muscle aldolase A messenger RNA
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