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J. Biol. Chem., Vol. 259, Issue 2, 797-804, 01, 1984

The covalent structure of hepatic microsomal epoxide hydrolase. II. The complete amino acid sequence

FS Heinemann and J Ozols

The complete amino acid sequence of hepatic microsomal epoxide hydrolase has been determined. The protein contains 455 amino acid residues in a single polypeptide chain and has Mr = 52,691. Peptides from selective chemical and proteolytic cleavages were isolated by a combination of gel filtration and high performance liquid chromatography and sequenced by automated Edman degradation. Overlapping peptide sequences were used to deduce the complete sequence. This is the first epoxide hydrolase and the third microsomal enzyme for which the complete sequence has been determined.
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