| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 259, Issue 2, 805-814, 01, 1984
JW Chase, JJ L'Italien, JB Murphy, EK Spicer and KR Williams
The ssb-113 (formerly lexC113) gene encoding a mutant single-stranded DNA
binding protein (SSB) has been cloned into plasmid pSC101 resulting in 5-
to 10-fold more mutant protein than strains carrying only one (chromosomal)
copy of the gene. Analysis of tryptic and chymotryptic peptides of the
mutant protein by high pressure liquid chromatography and solid phase
protein sequencing has shown that the ssb-113 mutation results in the
substitution of serine for proline at residue 176 of SSB. This change could
only occur in one step by a C leads to T transition in the DNA sequence.
Physicochemical studies of the homogeneous mutant protein have shown that
it binds as well as wild type SSB to single-stranded DNA and that it is a
slightly better helix- destabilizing protein than wild type SSB as measured
by its ability to lower the thermal melting transition of poly[d(A-T)]. In
vivo studies of ssb-113 strains carrying the cloned ssb-113 gene in pSC101
have shown that overproduction of the mutant protein does not complement
the temperature-sensitive conditional lethality caused by the ssb-113
mutation when present in single gene copy in contrast to effects recently
observed in ssb-1 strains overproducing the ssb-1 encoded protein (Chase,
J. W., Murphy, J. B., Whittier, R. F., Lorensen, E., and Sninsky, J. J.
(1983) J. Mol. Biol. 164, 193-211). Also noted in this report are two
corrections to the DNA sequence of wild type SSB, one of which places
glycine (codon GGC) at residue 133 rather than serine as previously
reported (Sancar, A., Williams, K. R., Chase, J. W., and Rupp, W. D. (1981)
Proc. Natl. Acad. Sci. U.S.A. 78, 4274- 4278). The second correction to the
DNA sequence is in the serine 39 codon, previously reported to be TCA and
now correctly shown to be TCC.
Characterization of the Escherichia coli SSB-113 mutant single-stranded DNA-binding protein. Cloning of the gene, DNA and protein sequence analysis, high pressure liquid chromatography peptide mapping, and DNA- binding studies
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Lu and J. L. Keck Structural basis of Escherichia coli single-stranded DNA-binding protein stimulation of exonuclease I PNAS, July 8, 2008; 105(27): 9169 - 9174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Arad, A. Hendel, C. Urbanke, U. Curth, and Z. Livneh Single-stranded DNA-binding Protein Recruits DNA Polymerase V to Primer Termini on RecA-coated DNA J. Biol. Chem., March 28, 2008; 283(13): 8274 - 8282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Witte, R. Fedorov, and U. Curth Biophysical Analysis of Thermus aquaticus Single-Stranded DNA Binding Protein Biophys. J., March 15, 2008; 94(6): 2269 - 2279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Shereda, D. A. Bernstein, and J. L. Keck A Central Role for SSB in Escherichia coli RecQ DNA Helicase Function J. Biol. Chem., June 29, 2007; 282(26): 19247 - 19258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Fedorov, G. Witte, C. Urbanke, D. J. Manstein, and U. Curth 3D structure of Thermus aquaticus single-stranded DNA-binding protein gives insight into the functioning of SSB proteins Nucleic Acids Res., December 5, 2006; (2006) gkl1002v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Y. Huen, X.-t. Li, L.-Y. Lu, R. M. Watt, D.-P. Liu, and J.-D. Huang The involvement of replication in single stranded oligonucleotide-mediated gene repair Nucleic Acids Res., December 4, 2006; 34(21): 6183 - 6194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. K. Davydova and L. B. Rothman-Denes Escherichia coli single-stranded DNA-binding protein mediates template recycling during transcription by bacteriophage N4 virion RNA polymerase PNAS, August 5, 2003; 100(16): 9250 - 9255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Witte, C. Urbanke, and U. Curth DNA polymerase III {chi} subunit ties single-stranded DNA binding protein to the bacterial replication machinery Nucleic Acids Res., August 1, 2003; 31(15): 4434 - 4440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bzymek and S. T. Lovett Instability of repetitive DNA sequences: The role of replication in multiple mechanisms PNAS, July 17, 2001; 98(15): 8319 - 8325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Li and R. S. Williams Tetramerization and Single-stranded DNA Binding Properties of Native and Mutated Forms of Murine Mitochondrial Single-stranded DNA-binding Proteins J. Biol. Chem., March 28, 1997; 272(13): 8686 - 8694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. M. Rehrauer, P. E. Lavery, E. L. Palmer, R. N. Singh, and S. C. Kowalczykowski Interaction of Escherichia coli RecA Protein with LexA Repressor. I. LexA REPRESSOR CLEAVAGE IS COMPETITIVE WITH BINDING OF A SECONDARY DNA MOLECULE J. Biol. Chem., September 27, 1996; 271(39): 23865 - 23873. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Markiewicz, C Malone, J W Chase, and L B Rothman-Denes Escherichia coli single-stranded DNA-binding protein is a supercoiled template-dependent transcriptional activator of N4 virion RNA polymerase. Genes & Dev., October 1, 1992; 6(10): 2010 - 2019. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
