| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 259, Issue 20, 12364-12367, 10, 1984
L Persson and AE Pegg
A specific antiserum to rat liver spermidine/spermine N1- acetyltransferase was used to study the induction of this protein. The antiserum had no effect on the spermidine acetylating capacity of crude nuclear extracts and very little effect on the activity present in crude cytosolic extracts from control rat tissues indicating that most of this activity is not due to spermidine/spermine N1- acetyltransferase. Treatment of rats with carbon tetrachloride, spermidine, thioacetamide, or methylglyoxal bis(guanylhydrazone) produced a substantial increase in the spermidine acetylating capacity of rat liver cytosolic extracts which was exclusively due to an increase in the immunoprecipitable spermidine/spermine N1- acetyltransferase protein. Exact measurement of the extent of this increase was not possible because the basal amount was too low to determine precisely but the amount of this enzyme increased about 250- fold with 6 h of treatment with carbon tetrachloride, about 25-fold at 6 h after spermidine, about 23-fold at 24 h after thioacetamide and up to 300-fold at 24 h after methylglyoxal bis(guanylhydrazone). Treatment of rats with spermidine also increased spermidine/spermine N1- acetyltransferase in other tissues including lung, kidney, and pancreas. The spermidine/spermine N1-acetyltransferase protein was found to turn over very rapidly with a half-life of about 15 min in thioacetamide-treated rats and 180 min after carbon tetrachloride.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. E. Pegg Spermidine/spermine-N1-acetyltransferase: a key metabolic regulator Am J Physiol Endocrinol Metab, June 1, 2008; 294(6): E995 - E1010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Marverti, M. G. Monti, A. E. Pegg, D. E. McCloskey, S. Bettuzzi, A. Ligabue, A. Caporali, D. D'Arca, and M. S. Moruzzi Spermidine/spermine N1-acetyltransferase transient overexpression restores sensitivity of resistant human ovarian cancer cells to N1,N12-bis(ethyl)spermine and to cisplatin Carcinogenesis, October 1, 2005; 26(10): 1677 - 1686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. M. Bras, J. Jänne, C. W. Porter, and D. S. Sitar Spermidine/Spermine N1-Acetyltransferase Catalyzes Amantadine Acetylation Drug Metab. Dispos., April 13, 2001; 29(5): 676 - 680. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Merali Pneumocystis carinii Polyamine Catabolism J. Biol. Chem., July 23, 1999; 274(30): 21017 - 21022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Coleman and A. E. Pegg Proteasomal Degradation of Spermidine/Spermine N1-Acetyltransferase Requires the Carboxyl-terminal Glutamic Acid Residues J. Biol. Chem., May 2, 1997; 272(18): 12164 - 12169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lu, K. A. Berkey, and R. A. Casero Jr. RGFGIGS Is an Amino Acid Sequence Required for Acetyl Coenzyme A Binding and Activity of Human Spermidine/Spermine N1Acetyltransferase J. Biol. Chem., August 2, 1996; 271(31): 18920 - 18924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-i. Fukuchi, K. Kashiwagi, M. Yamagishi, A. Ishihama, and K. Igarashi Decrease in Cell Viability Due to the Accumulation of Spermidine in Spermidine Acetyltransferase-deficient Mutant of Escherichia coli J. Biol. Chem., August 11, 1995; 270(32): 18831 - 18835. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
