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J. Biol. Chem., Vol. 259, Issue 21, 12997-13003, 11, 1984

A kinetic analysis of the interaction of elongation factor Tu with guanosine nucleotides and elongation factor Ts

JF Eccleston

The interaction of elongation factor Tu (EF-Tu) and elongation factor Ts (EF-Ts) from Escherichia coli has been investigated by kinetic methods. It was found that EF-Ts purified on an EF-Tu affinity column contained a transphosphorylase activity which could transfer the gamma- phosphate of GTP to [3H]GDP. However, this activity showed different sensitivities to heat and N-ethylmaleimide compared to the EF-Ts activity. Using the chromophoric GDP analogue, 2-amino-6-mercaptopurine riboside 5'-diphosphate (thioGDP), spectrophotometric titrations and stopped-flow experiments enabled the interaction of EF-Tu X thioGDP with EF-Ts and of EF-Tu X EF-Ts with thioGDP to be investigated. The results were analyzed according to the scheme of Chau et al. (Chau, V., Romero, G., and Biltonen, R.L. (1981) J. Biol. Chem. 256, 5591-5596). (Formula: see text) Values for the rate constants obtained were k1 greater than or equal to 2 X 10(8) M-1 s-1, k-1 greater than or equal to 2600 s-1, k2 = 500 s-1, and k-2 = 4 X 10(5) M-1 s-1. The most notable feature of these results is that EF-Ts binds to EF-Tu X thioGDP at a rate approaching that expected for a diffusion-controlled reaction whereas thioGDP binds to EF-Tu X EF-Ts several orders of magnitude more slowly than this. The relevance of these results to the interactions involving GDP is discussed.
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