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J. Biol. Chem., Vol. 259, Issue 22, 13730-13736, Nov, 1984
Y Nakabeppu, T Miyata, H Kondo, S Iwanaga and M Sekiguchi
Nucleotide sequence of a DNA fragment containing the alkA gene and its
control region has been determined using a chemical method. Only one open
reading frame responsible for 3-methyladenine DNA glycosylase II was found.
The hypothetical polypeptide deduced from the DNA sequence, with a
molecular weight of 31,400, has an amino-terminal sequence and total amino
acid composition identical to that of purified 3- methyladenine DNA
glycosylase II. We constructed hybrid plasmids carrying an alkA'-lacZ'
fusion, with the proper control region for alkA expression. A hybrid
polypeptide with beta-galactosidase activity was formed when lac mutant
cells harboring such plasmids were incubated with low doses of
N-methyl-N'-nitro-N-nitrosoguanidine or methylmethane sulfonate. Other
DNA-damaging agents, such as ethylmethane sulfonate, nalidixic acid, and
ultraviolet light did not induce the enzyme activity. The induction was
controlled by the ada and adc, but not by the recA and lexA genes.
Structure and expression of the alkA gene of Escherichia coli involved in adaptive response to alkylating agents
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