JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hemmings, H. C.
Right arrow Articles by Greengard, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hemmings, H. C., Jr
Right arrow Articles by Greengard, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J. Biol. Chem., Vol. 259, Issue 23, 14491-14497, 12, 1984

DARPP-32, a dopamine- and adenosine 3':5'-monophosphate-regulated neuronal phosphoprotein. II. Comparison of the kinetics of phosphorylation of DARPP-32 and phosphatase inhibitor 1

HC Hemmings Jr, AC Nairn and P Greengard

DARPP-32 (dopamine- and cyclic AMP-regulated phosphoprotein, Mr = 32,000) is a cytosolic neuronal phosphoprotein enriched in dopamine- innervated brain regions which, in its phosphorylated form, acts as an inhibitor of protein phosphatase 1. We have compared the phosphorylation of purified DARPP-32 with that of purified phosphatase inhibitor 1, a widespread inhibitor of protein phosphatase 1. Purified cyclic AMP-dependent protein kinase and cyclic GMP-dependent protein kinase each catalyzed the maximal incorporation of 0.9-1.1 mol of [32P]phosphate/mol of DARPP-32 or phosphatase inhibitor 1, with phosphorylation occurring on threonine residues. Evidence for the existence of a single phosphorylation site in each substrate protein was obtained by two-dimensional thin-layer phosphopeptide mapping of thermolytic digests. Initial rate studies of the phosphorylation of DARPP-32 yielded an apparent Km of 2.4 microM and a kcat of 2.7 S-1 for the catalytic subunit of cyclic AMP-dependent protein kinase, and an apparent Km of 5.4 microM and a kcat of 2.3 S-1 for cyclic GMP- dependent protein kinase. These in vitro results are compatible with a physiological role for the phosphorylation of DARPP-32 by either protein kinase in vivo. Similar studies with phosphatase inhibitor 1 yielded an apparent Km of 5.0 microM and a kcat of 1.4 S-1 for the catalytic subunit of cyclic AMP-dependent protein kinase, and an apparent Km of 25.0 microM and a kcat of 1.2 S-1 for cyclic GMP- dependent protein kinase. A synthetic nonapeptide, corresponding to the phosphorylation site of DARPP-32, was phosphorylated with apparent Km values of 1.12 mM and 1.86 mM and kcat values of 0.22 S-1 and 3.4 S-1 for cyclic AMP-dependent and cyclic GMP-dependent protein kinase, respectively.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. G. Ni, N. Wang, D. J. Cao, N. Sachan, D. J. Morris, R. D. Gerard, M. Kuro-o, B. A. Rothermel, and J. A. Hill
FoxO transcription factors activate Akt and attenuate insulin signaling in heart by inhibiting protein phosphatases
PNAS, December 18, 2007; 104(51): 20517 - 20522.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Nguyen, A. Nishi, J. W. Kansy, J. Fernandez, K. Hayashi, F. Gillardon, H. C. Hemmings Jr., A. C. Nairn, and J. A. Bibb
Regulation of Protein Phosphatase Inhibitor-1 by Cyclin-dependent Kinase 5
J. Biol. Chem., June 1, 2007; 282(22): 16511 - 16520.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. F. Cooke, J. Wu, F. Plattner, M. Errington, M. Rowan, M. Peters, A. Hirano, K. D. Bradshaw, R. Anwyl, T. V. P. Bliss, et al.
Autophosphorylation of {alpha}CaMKII is not a general requirement for NMDA receptor-dependent LTP in the adult mouse
J. Physiol., August 1, 2006; 574(3): 805 - 818.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. S. Eyny and J. C. Horvitz
Opposing Roles of D1 and D2 Receptors in Appetitive Conditioning
J. Neurosci., March 1, 2003; 23(5): 1584 - 1587.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Dobransky, D. Brewer, G. Lajoie, and R. J. Rylett
Phosphorylation of 69-kDa Choline Acetyltransferase at Threonine 456 in Response to Amyloid-beta Peptide 1-42
J. Biol. Chem., February 14, 2003; 278(8): 5883 - 5893.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. Calabresi, P. Gubellini, D. Centonze, B. Picconi, G. Bernardi, K. Chergui, P. Svenningsson, A. A. Fienberg, and P. Greengard
Dopamine and cAMP-Regulated Phosphoprotein 32 kDa Controls Both Striatal Long-Term Depression and Long-Term Potentiation, Opposing Forms of Synaptic Plasticity
J. Neurosci., November 15, 2000; 20(22): 8443 - 8451.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. S. Ozer and S. Halpain
Phosphorylation-dependent Localization of Microtubule-associated Protein MAP2c to the Actin Cytoskeleton
Mol. Biol. Cell, October 1, 2000; 11(10): 3573 - 3587.
[Abstract] [Full Text]

Home page
 Physiol. Rev.Home page
S. Herzig and J. Neumann
Effects of Serine/Threonine Protein Phosphatases on Ion Channels in Excitable Membranes
Physiol Rev, January 1, 2000; 80(1): 173 - 210.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. U. Hall, S. P. Collins, D. M. Gamm, E. Massa, A. A. DePaoli-Roach, and M. D. Uhler
Phosphorylation-dependent Inhibition of Protein Phosphatase-1 by G-substrate. A PURKINJE CELL SUBSTRATE OF THE CYCLIC GMP-DEPENDENT PROTEIN KINASE
J. Biol. Chem., February 5, 1999; 274(6): 3485 - 3495.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. le Gouvello, V. Manceau, and A. Sobel
Serine 16 of Stathmin as a Cytosolic Target for Ca2+/Calmodulin-Dependent Kinase II After CD2 Triggering of Human T Lymphocytes
J. Immunol., August 1, 1998; 161(3): 1113 - 1122.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. L. Andress
Insulin-like growth factor-binding protein-5 (IGFBP-5) stimulates phosphorylation of the IGFBP-5 receptor
Am J Physiol Endocrinol Metab, April 1, 1998; 274(4): E744 - E750.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Blank, I. Nijholt, U. Teichert, H. Kugler, H. Behrsing, A. Fienberg, P. Greengard, and J. Spiess
The phosphoprotein DARPP-32 mediates cAMP-dependent potentiation of striatal N-methyl-D-aspartate responses
PNAS, December 23, 1997; 94(26): 14859 - 14864.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-Y. Yun, S. L. Milgram, H. T. Keutmann, and B. A. Eipper
Phosphorylation of the Cytosolic Domain of Peptidylglycine alpha-Amidating Monooxygenase
J. Biol. Chem., December 15, 1995; 270(50): 30075 - 30083.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F.édér. Desdouits, D. Cohen, A. C. Nairn, P. Greengard, and J.-A. Girault
Phosphorylation of DARPP-32, a Dopamine- and cAMP-regulated Phosphoprotein, by Casein Kinase I in Vitro and in Vivo
J. Biol. Chem., April 14, 1995; 270(15): 8772 - 8778.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Yang, T. D. Hurley, and A. A. DePaoli-Roach
Interaction of Inhibitor-2 with the Catalytic Subunit of Type 1 Protein Phosphatase. IDENTIFICATION OF A SEQUENCE ANALOGOUS TO THE CONSENSUS TYPE 1 PROTEIN PHOSPHATASE-BINDING MOTIF
J. Biol. Chem., July 21, 2000; 275(30): 22635 - 22644.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1984 by the American Society for Biochemistry and Molecular Biology.