| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 259, Issue 4, 2051-2054, 02, 1984
S Braun, WE Raymond and E Racker
Several synthetic random polymers of tyrosine containing glutamic acid, alanine, and lysine in various proportion served as substrates for tyrosine-specific protein kinases. The Km values for these substrates were much lower than for small polypeptides such as angiotensin. For the protein kinase coded by Fujinami virus, the best substrates (with the lowest Km) were polymers containing glutamic acid, alanine, and 8 to 10% tyrosine; for the insulin receptor protein kinase, the best substrate was a polymer containing 80% glutamic acid and 20% tyrosine. These polymers serve as inexpensive and tyrosine-specific substrates that can be used even with crude extracts and analyzed by the convenient filter paper assay. Several synthetic polymers with ordered sequences were found to be potent inhibitors of these tyrosine-specific protein kinases.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. H. Sim, D. S. S. Lio, S. S. Mok, C. L. Masters, A. F. Hill, J. G. Culvenor, and H.-C. Cheng C-terminal truncation and Parkinson's disease-associated mutations down-regulate the protein serine/threonine kinase activity of PTEN-induced kinase-1 Hum. Mol. Genet., November 1, 2006; 15(21): 3251 - 3262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tardif, C. Dube, S. Chevalier, and J. L. Bailey Capacitation Is Associated with Tyrosine Phosphorylation and Tyrosine Kinase-Like Activity of Pig Sperm Proteins Biol Reprod, September 1, 2001; 65(3): 784 - 792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Nichols and B. H. Morimoto Differential Inhibition of Multiple cAMP Phosphodiesterase Isozymes by Isoflavones and Tyrphostins Mol. Pharmacol., April 1, 2000; 57(4): 738 - 745. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Ueki, S. Mitsugi, Y. Kawashima, T. Motoyashiki, and T. Morita Orthovanadate Stimulates Cyclic Guanosine Monophosphate-Inhibited Cyclic Adenosine Monophosphate Phosphodiesterase Activity in Isolated Rat Fat Pads through Activation of Particulate Myelin Basic Protein Kinase by Protein Tyrosine Kinase Endocrinology, July 1, 1997; 138(7): 2784 - 2789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S G Grant, K A Karl, M A Kiebler, and E R Kandel Focal adhesion kinase in the brain: novel subcellular localization and specific regulation by Fyn tyrosine kinase in mutant mice. Genes & Dev., August 1, 1995; 9(15): 1909 - 1921. [Abstract] [PDF]
|
|
|
|
 |
|
 P Yaish, A Gazit, C Gilon, and A Levitzki Blocking of EGF-dependent cell proliferation by EGF receptor kinase inhibitors Science, November 11, 1988; 242(4880): 933 - 935. [Abstract] [PDF]
|
|
|
|
|
|
G Schieven, J Thorner, and G. Martin Protein-tyrosine kinase activity in Saccharomyces cerevisiae Science, January 24, 1986; 231(4736): 390 - 393. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
