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J. Biol. Chem., Vol. 259, Issue 4, 2100-2107, 02, 1984
KL Reue, DH Quon, KA O'Donnell, GJ Dizikes, GC Fareed and AJ Lusis
A cDNA clone for mouse apolipoprotein E has been identified from a mouse
liver cDNA library by a combination of differential colony hybridization
and hybrid selection-translation. The identity of the clone was
unambiguously established by partial sequencing and comparison with human
apolipoprotein E nucleotide and amino acid sequences. In conjunction with
an in vitro translation assay for apolipoprotein E, the clone has been used
to examine the relative levels of apolipoprotein E mRNA in various tissues
of the mouse and the regulation of apolipoprotein E synthesis in response
to a diet rich in saturated fat and cholesterol. In the tissues examined,
the clone was found to hybridize to a polyadenylated RNA species of
approximately 1400 nucleotides. Of the tissues involved in lipoprotein
synthesis, liver is very rich (about 1% of total) in apolipoprotein E mRNA
while intestine contains only trace amounts. Appreciable levels of active
apolipoprotein E mRNA (up to 10% of that in liver) are also detected in
peripheral tissues not associated with lipoprotein synthesis, including
lung, kidney, spleen, and heart. Thus, extrahepatic apolipoprotein E
synthesis may contribute significantly to the levels present in plasma, and
a possible function in "reverse cholesterol transport" is considered. When
mice were placed on a high lipid diet there was no discernible change in
the level of apolipoprotein E mRNA in liver or intestine, although the
level of the circulating protein increased about 3-fold. We conclude that
in mice the effect of diet on apolipoprotein E levels in blood does not
result from induction of mRNA in these tissues.
Cloning and regulation of messenger RNA for mouse apolipoprotein E
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