J. Biol. Chem., Vol. 259, Issue 6, 3639-3643, Mar, 1984
Radiochemical determination of polyamines in poliovirus and human rhinovirus 14
GS Fout, KC Medappa, JE Mapoles and RR Rueckert
HeLa cells were made strictly dependent upon polyamine by growth in the
presence of alpha-difluoromethylornithine, a specific inhibitor of
ornithine decarboxylase. Under these conditions, the specific activity of
the cellular polyamine pools eventually equilibrated to that of exogenously
supplied [14C]putrescine; however, the process was very slow, requiring
half-equilibration times of about 16 h for spermidine and 28 for spermine.
Thus, the distribution of radioactivity in individual polyamines became a
valid measure of polyamine content only after a continuous 4-day
incorporation period. When propagated in polyamine-labeled cells, two
picornaviruses were found to incorporate substantially different amounts of
polyamine: about 0.6% of the cell pool for human rhinovirus 14 but only
0.04% for poliovirus. This content of polyamine was sufficient to
neutralize nearly 27% of the negative charge of the RNA in human rhinovirus
14 but only 1.6% in poliovirus.
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