| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 259, Issue 9, 5430-5439, May, 1984
WA Marasco, SH Phan, H Krutzsch, HJ Showell, DE Feltner, R Nairn, EL Becker and PA Ward
Chemotactic factor-enriched butanol extracts from Escherichia coli culture
filtrates were fractionated and purified by high pressure liquid
chromatography. The yield from individual fractions of biological activity
(lysosomal enzyme secretion) and antigenic activity (competition with
[3H]fMet-Leu-Phe for binding to rabbit anti-fMet-Leu- Phe) revealed an
average 50% recovery of original material. Five peaks of biological
activity were separated as demonstrated by enzyme- releasing activity.
Three of these peaks coincided exactly with peaks of antigenic activity,
suggesting that at least 3 and as many as 5 distinct formyl-methionyl
peptides had been separated. The majority of recovered activity appeared in
peak 3 and represented 70% of the total biological and antigenic activities
recovered. The five peak fractions were subsequently analyzed by dipeptidyl
carboxypeptidase gas chromatography-mass spectrometry (DCP/GC-MS) to
determine amino acid sequences. After digestion, the formyl-Met peptide was
demonstrated in only one of the five peak fractions (peak 3). Furthermore,
both the GC retention times and mass spectra indicated that peak 3
contained formyl- methionyl-leucyl-phenylalanine. The DCP/GC and MS data
were confirmed with tests made on authentic fMet-Leu-Phe. Butanol extracts
from E. coli filtrates to which were added synthetic fMet-Leu-Phe resulted
in increased biological and antigenic activity in the precise high pressure
liquid chromatography fractions of peak 3 where the fMet-Leu- Phe produced
by E. coli was found. Finally, the analysis of recovered biological and
antigenic activities indicated that the formyl peptides were found in
nanomolar concentrations in culture filtrates. These results demonstrate
that the NH2-terminal formyl peptides produced by E. coli, of which
formyl-methionyl-leucyl-phenylalanine appears to be the major component,
are the peptide mediators responsible for leukocyte chemotactic activity in
the bacterial culture extracts.
Purification and identification of formyl-methionyl-leucyl- phenylalanine as the major peptide neutrophil chemotactic factor produced by Escherichia coli
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. L. Southgate, R. L. He, J.-L. Gao, P. M. Murphy, M. Nanamori, and R. D. Ye Identification of Formyl Peptides from Listeria monocytogenes and Staphylococcus aureus as Potent Chemoattractants for Mouse Neutrophils J. Immunol., July 15, 2008; 181(2): 1429 - 1437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Babbin, A. J. Jesaitis, A. I. Ivanov, D. Kelly, M. Laukoetter, P. Nava, C. A. Parkos, and A. Nusrat Formyl Peptide Receptor-1 Activation Enhances Intestinal Epithelial Cell Restitution through Phosphatidylinositol 3-Kinase-Dependent Activation of Rac1 and Cdc42 J. Immunol., December 15, 2007; 179(12): 8112 - 8121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Pinho, R. de Castro Russo, F. A. Amaral, L. P. de Sousa, M. M. Barsante, D. G. de Souza, J. C. Alves-Filho, D. C. Cara, J. S. Hayflick, C. Rommel, et al. Tissue- and Stimulus-Dependent Role of Phosphatidylinositol 3-Kinase Isoforms for Neutrophil Recruitment Induced by Chemoattractants In Vivo J. Immunol., December 1, 2007; 179(11): 7891 - 7898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Viswanathan, R. G. Painter, N. A. Lanson Jr., and G. Wang Functional Expression of N-Formyl Peptide Receptors in Human Bone Marrow-Derived Mesenchymal Stem Cells Stem Cells, May 1, 2007; 25(5): 1263 - 1269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. John, V. Sahni, D. Mehet, J. F. Morris, H. C. Christian, M. Perretti, R. J. Flower, E. Solito, and J. C. Buckingham Formyl peptide receptors and the regulation of ACTH secretion: targets for annexin A1, lipoxins, and bacterial peptides FASEB J, April 1, 2007; 21(4): 1037 - 1046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Carlson, S. R. Vavricka, J. J. Eloranta, M. W. Musch, D. L. Arvans, K. A. Kles, M. M. Walsh-Reitz, G. A. Kullak-Ublick, and E. B. Chang fMLP induces Hsp27 expression, attenuates NF-{kappa}B activation, and confers intestinal epithelial cell protection Am J Physiol Gastrointest Liver Physiol, April 1, 2007; 292(4): G1070 - G1078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Edwards, C. Bologa, S. M. Young, K. V. Balakin, E. R. Prossnitz, N. P. Savchuck, L. A. Sklar, and T. I. Oprea Integration of Virtual Screening with High-Throughput Flow Cytometry to Identify Novel Small Molecule Formylpeptide Receptor Antagonists Mol. Pharmacol., November 1, 2005; 68(5): 1301 - 1310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-J. Haas, C. J. C. de Haas, W. Kleibeuker, M. J. J. G. Poppelier, K. P. M. van Kessel, J. A. W. Kruijtzer, R. M. J. Liskamp, and J. A. G. van Strijp N-Terminal Residues of the Chemotaxis Inhibitory Protein of Staphylococcus aureus Are Essential for Blocking Formylated Peptide Receptor but Not C5a Receptor J. Immunol., November 1, 2004; 173(9): 5704 - 5711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Le, P. Iribarren, Y. Zhou, W. Gong, J. Hu, X. Zhang, and J. M. Wang Silencing the Formylpeptide Receptor FPR by Short-Interfering RNA Mol. Pharmacol., October 1, 2004; 66(4): 1022 - 1028. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Frohlich, S. Wittmann, G. Rothe, D. I. Sessler, P. Vogel, and K. Taeger Mild Hyperthermia Down-Regulates Receptor-Dependent Neutrophil Function Anesth. Analg., July 1, 2004; 99(1): 284 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Partida-Sanchez, P. Iribarren, M. E. Moreno-Garcia, J.-L. Gao, P. M. Murphy, N. Oppenheimer, J. M. Wang, and F. E. Lund Chemotaxis and Calcium Responses of Phagocytes to Formyl Peptide Receptor Ligands Is Differentially Regulated by Cyclic ADP Ribose J. Immunol., February 1, 2004; 172(3): 1896 - 1906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Song, S Suzuki, H Kubo, M Chida, Y Hoshikawa, T Tabata, and T Kondo Effects of antiplatelet agents on pulmonary haemodynamic response to fMLP in endotoxin primed rats Thorax, January 1, 2004; 59(1): 39 - 44. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Buyse, L. Charrier, S. Sitaraman, A. Gewirtz, and D. Merlin Interferon-{gamma} Increases hPepT1-Mediated Uptake of Di-Tripeptides Including the Bacterial Tripeptide fMLP in Polarized Intestinal Epithelia Am. J. Pathol., November 1, 2003; 163(5): 1969 - 1977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Peters, R. E. Unger, J. Brunner, and C.J. Kirkpatrick Molecular basis of endothelial dysfunction in sepsis Cardiovasc Res, October 15, 2003; 60(1): 49 - 57. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Fu, C. Dahlgren, and J. Bylund Subinhibitory Concentrations of the Deformylase Inhibitor Actinonin Increase Bacterial Release of Neutrophil-Activating Peptides: a New Approach to Antimicrobial Chemotherapy Antimicrob. Agents Chemother., August 1, 2003; 47(8): 2545 - 2550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Buyse, A. Tsocas, F. Walker, D. Merlin, and A. Bado PepT1-mediated fMLP transport induces intestinal inflammation in vivo Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1795 - C1800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Svensson, C. Dahlgren, and C. Wenneras The chemoattractant Trp-Lys-Tyr-Met-Val-D-Met activates eosinophils through the formyl peptide receptor and one of its homologues, formyl peptide receptor-like 1 J. Leukoc. Biol., October 1, 2002; 72(4): 810 - 818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Vaughn, R. J. Proske, and D. L. Haviland Identification, Cloning, and Functional Characterization of a Murine Lipoxin A4 Receptor Homologue Gene J. Immunol., September 15, 2002; 169(6): 3363 - 3369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Yang, Q. Chen, B. Gertz, R. He, M. Phulsuksombati, R. D. Ye, and J. J. Oppenheim Human dendritic cells express functional formyl peptide receptor-like-2 (FPRL2) throughout maturation J. Leukoc. Biol., September 1, 2002; 72(3): 598 - 607. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Schroder, R. Hasler, J. Grabowsky, B. Kahlke, and A. I. Mallet Identification of Diacylated Ureas as a Novel Family of Fungus-specific Leukocyte-activating Pathogen-associated Molecules J. Biol. Chem., July 26, 2002; 277(31): 27887 - 27895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Barabe, G. Pare, M. J. G. Fernandes, S. G. Bourgoin, and P. H. Naccache Cholesterol-modulating Agents Selectively Inhibit Calcium Influx Induced by Chemoattractants in Human Neutrophils J. Biol. Chem., April 12, 2002; 277(16): 13473 - 13478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Grandel, M. Reutemann, L. Kiss, M. Buerke, L. Fink, E. Bournelis, M. Heep, W. Seeger, F. Grimminger, and U. Sibelius Staphylococcal alpha -toxin provokes neutrophil-dependent cardiac dysfunction: role of ICAM-1 and cys-leukotrienes Am J Physiol Heart Circ Physiol, March 1, 2002; 282(3): H1157 - H1165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sodhi and S. K. Biswas fMLP-induced in vitro nitric oxide production and its regulation in murine peritoneal macrophages J. Leukoc. Biol., February 1, 2002; 71(2): 262 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Matute-Bello, C. W. Frevert, W. C. Liles, M. Nakamura, J. T. Ruzinski, K. Ballman, V. A. Wong, C. Vathanaprida, and T. R. Martin Fas/Fas Ligand System Mediates Epithelial Injury, but Not Pulmonary Host Defenses, in Response to Inhaled Bacteria Infect. Immun., September 1, 2001; 69(9): 5768 - 5776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. LEAKEY, R. HIRST, and J. LA BROOY A low molecular weight factor is a significant mediator of non-opsonic neutrophil activation by Helicobacter pylori J. Med. Microbiol., September 1, 2001; 50(9): 787 - 794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. Hu, Y. Le, W. Gong, N. M. Dunlop, J. L. Gao, P. M. Murphy, and J. M. Wang Synthetic peptide MMK-1 is a highly specific chemotactic agonist for leukocyte FPRL1 J. Leukoc. Biol., July 1, 2001; 70(1): 155 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Lauvau and E. G. Pamer CD8 T Cell Detection of Bacterial Infection: Sniffing for Formyl Peptides Derived from Mycobacterium tuberculosis J. Exp. Med., May 21, 2001; 193(10): F35 - F40. [Full Text] [PDF]
|
|
|
|
|
|
I. E. Nwakoby, K. Reddy, P. Patel, N. Shah, S. Sharma, M. Bhaskaran, N. Gibbons, A. A. Kapasi, and P. C. Singhal Fas-Mediated Apoptosis of Neutrophils in Sera of Patients with Infection Infect. Immun., May 1, 2001; 69(5): 3343 - 3349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Biondi, B. Pavan, M. Enrica Ferretti, F. Ginanni Corradini, L. Maria Neri, and F. Vesce Formyl-Methionyl-Leucyl-Phenylalanine Induces Prostaglandin E2 Release from Human Amnion-Derived WISH Cells by Phospholipase C-Mediated [Ca2+]i Rise Biol Reprod, March 1, 2001; 64(3): 865 - 870. [Abstract] [Full Text]
|
|
|
|
|
|
K. Hattar, U. Sibelius, A. Bickenbach, E. Csernok, W. Seeger, and F. Grimminger Subthreshold concentrations of anti-proteinase 3 antibodies (c-ANCA) specifically prime human neutrophils for fMLP-induced leukotriene synthesis and chemotaxis J. Leukoc. Biol., January 1, 2001; 69(1): 89 - 97. [Abstract] [Full Text]
|
|
|
|
|
|
R. He, L. Tan, D. D. Browning, J. M. Wang, and R. D. Ye The Synthetic Peptide Trp-Lys-Tyr-Met-Val-D-Met Is a Potent Chemotactic Agonist for Mouse Formyl Peptide Receptor J. Immunol., October 15, 2000; 165(8): 4598 - 4605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Heine, K. Jaeger, A. Osthaus, N. Weingaertner, S. Munte, S. Piepenbrock, and M. Leuwer Anaesthesia with propofol decreases FMLP-induced neutrophil respiratory burst but not phagocytosis compared with isoflurane Br. J. Anaesth., September 1, 2000; 85(3): 424 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. Martin Recognition of Bacterial Endotoxin in the Lungs Am. J. Respir. Cell Mol. Biol., August 1, 2000; 23(2): 128 - 132. [Full Text]
|
|
|
|
|
|
J. K. Hartt, G. Barish, P. M. Murphy, and J.-L. Gao N-formylpeptides Induce Two Distinct Concentration Optima for Mouse Neutrophil Chemotaxis by Differential Interaction with Two N-formylpeptide Receptor (FPR) Subtypes: Molecular Characterization of FPR2, a Second Mouse Neutrophil FPR J. Exp. Med., September 6, 1999; 190(5): 741 - 748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Buzzi, F. Vesce, M. E. Ferretti, E. Fabbri, and C. Biondi Does Formyl-Methionyl-Leucyl-Phenylalanine Exert a Physiological Role in Labor in Women? Biol Reprod, May 1, 1999; 60(5): 1211 - 1216. [Abstract] [Full Text]
|
|
|
|
|
|
H. A. Edens, C. A. Parkos, T. W. Liang, A. J. Jesaitis, J. E. Cutler, and H. M. Miettinen Non-Serum-Dependent Chemotactic Factors Produced by Candida albicans Stimulate Chemotaxis by Binding to the Formyl Peptide Receptor on Neutrophils and to an Unknown Receptor on Macrophages Infect. Immun., March 1, 1999; 67(3): 1063 - 1071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Gao, E. J. Lee, and P. M. Murphy Impaired Antibacterial Host Defense in Mice Lacking the N-formylpeptide Receptor J. Exp. Med., February 15, 1999; 189(4): 657 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Green, J. Trial, and H. H. Birdsall TNF-{alpha} Released by Comigrating Monocytes Promotes Transendothelial Migration of Activated Lymphocytes J. Immunol., September 1, 1998; 161(5): 2481 - 2489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Princiotta, L. L. Lenz, M. J. Bevan, and U. D. Staerz H2-M3 Restricted Presentation of a Listeria-derived Leader Peptide J. Exp. Med., May 18, 1998; 187(10): 1711 - 1719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Mills, H. M. Miettinen, D. Barnidge, M. J. Vlases, S. Wimer-Mackin, E. A. Dratz, J. Sunner, and A. J. Jesaitis Identification of a Ligand Binding Site in the Human Neutrophil Formyl Peptide Receptor Using a Site-specific Fluorescent Photoaffinity Label and Mass Spectrometry J. Biol. Chem., April 24, 1998; 273(17): 10428 - 10435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Anton, J O'Connell, D O'Connell, L Whitaker, G C O'Sullivan, J K Collins, and F Shanahan Mucosal subepithelial binding sites for the bacterial chemotactic peptide, formyl-methionyl-leucyl-phenylalanine (FMLP) Gut, March 1, 1998; 42(3): 374 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Sabeh, P. Hockberger, and M. M. Sayeed Signaling mechanisms of elevated neutrophil O−2 generation after burn injury Am J Physiol Regulatory Integrative Comp Physiol, February 1, 1998; 274(2): R476 - R485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Hinterleitner, J. D. Valentich, J.-H. Cha, P. Will, A. Welton, and D. W. Powell J. Pharmacol. Exp. Ther., June 1, 1997; 281(3): 1264 - 1271. [Abstract] [Full Text]
|
|
|
|
|
|
S. Raiden, M. Giordano, G. Andonegui, A. S. Trevani, D. H. López, V. Nahmod, and J. R. Geffner Losartan, a Selective Inhibitor of Subtype AT1 Receptors for Angiotensin II, Inhibits the Binding of N-Formylmethionyl-leucyl-phenylalanine to Neutrophil Receptors J. Pharmacol. Exp. Ther., May 1, 1997; 281(2): 624 - 628. [Abstract] [Full Text]
|
|
|
|
|
|
J. S. Mills, H. M. Miettinen, D. Cummings, and A. J. Jesaitis Characterization of the Binding Site on the Formyl Peptide Receptor Using Three Receptor Mutants and Analogs of Met-Leu-Phe and Met-Met-Trp-Leu-Leu J. Biol. Chem., December 8, 2000; 275(50): 39012 - 39017. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
