J. Biol. Chem., Vol. 259, Issue 9, 5549-5554, May, 1984
Distinctions between mechanisms of cytochalasin D activity for Mg2+- and K+-induced actin assembly
A Mozo-Villarias and BR Ware
The fraction of assembled actin and the diffusion coefficients of
filamentous and nonfilamentous species have been determined by fluorescence
photobleaching recovery. For both Mg2+-induced and K+- induced actin
assembly, a higher concentration of cation leads to longer filaments.
Cytochalasin D reduces the fraction of actin present as assembled
filaments. In the absence of Mg2+, the accompanying increase in diffusion
coefficient of the filaments is of an appropriate magnitude to be accounted
for by shortening of filaments as a result of net depolymerization. In the
presence of Mg2+, cytochalasin D induces a dose-dependent increase in
diffusion coefficient up to about a factor of 10. This increase indicates a
shortening of filaments consistent with extensive filament cleavage. Under
all conditions studied, the unassembled actin is present primarily as
monomer.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
