HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kreutter, D. Articles by Morin, M. J. Search for Related Content PubMed PubMed Citation Articles by Kreutter, D. Articles by Morin, M. J. Social Bookmarking                      What's this?

J. Biol. Chem., Vol. 260, Issue 10, 5979-5984, May, 1985

Dissociation of protein kinase C activation from phorbol ester-induced maturation of HL-60 leukemia cells

D Kreutter, AB Caldwell and MJ Morin

The role of C-kinase in the induction of maturation of HL-60 promyelocytic leukemia cells was examined using two activators of this kinase, 12-O-tetradecanoyl phorbol 13-acetate (TPA) and 1-oleoyl-2- acetylglycerol (OAG). At 10(-8) M, a concentration that induced maturation, TPA effectively stimulated C-kinase activity in cell-free preparations by increasing the affinity of the enzyme for Ca2+. Similar activation was observed with 20 micrograms/ml of OAG. At these concentrations, addition of either compound to intact cells stimulated the phosphorylation of cellular proteins. Treatment with TPA resulted in an increased phosphorylation of 14 proteins, 9 of which also changed in response to OAG. In addition to the effects on protein phosphorylation, TPA and OAG both affected choline lipid metabolism. TPA at 10(-8) M stimulated the incorporation of [methyl-3H]choline into phosphatidylcholine, sphingomyelin, and lysophosphatidylcholine. OAG at 20 micrograms/ml had quantitatively similar effects on the labeling of the former two lipids, but did not affect incorporation of choline into lysophosphatidylcholine. Despite the similar biochemical effects of TPA and OAG, the diglyceride was unable to induce HL-60 cell maturation as measured by inhibition of cell growth, development of nonspecific esterase activity, phagocytosis, adherence of cells to plastic, and loss of transferrin receptor activity. The lack of effect is not due to metabolism of OAG; maturation could not be induced by treating cells with fresh OAG every 2 h for a period of 12 h. These results suggest a dissociation of the activation of C-kinase and the induction of HL-60 cell maturation by TPA.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Giorgio, M. R. M. Santos, A. H. Straus, H. K. Takahashi, and C. L. Barbieri Effect of Glycosphingolipids Purified from Leishmania (Leishmania) amazonensis Amastigotes on Human Peripheral Lymphocytes Clin. Vaccine Immunol., May 1, 2003; 10(3): 469 - 472. [Abstract] [Full Text] [PDF]

				H. Seimiya and T. Tsuruo Functional Involvement of PTP-U2L in Apoptosis Subsequent to Terminal Differentiation of Monoblastoid Leukemia Cells J. Biol. Chem., August 14, 1998; 273(33): 21187 - 21193. [Abstract] [Full Text] [PDF]

				R. Manfredini, R. Balestri, E. Tagliafico, F. Trevisan, M. Pizzanelli, A. Grande, D. Barbieri, P. Zucchini, G. Citro, C. Franceschi, et al. Antisense Inhibition of c-fes Proto-oncogene Blocks PMA-Induced Macrophage Differentiation in HL60 and in FDC-P1/MAC-11 Cells Blood, January 1, 1997; 89(1): 135 - 145. [Abstract] [Full Text] [PDF]

				S. J. Slater, C. Ho, M. B. Kelly, J. D. Larkin, F. J. Taddeo, M. D. Yeager, and C. D. Stubbs Protein Kinase Calpha Contains Two Activator Binding Sites That Bind Phorbol Esters and Diacylglycerols with Opposite Affinities J. Biol. Chem., March 1, 1996; 271(9): 4627 - 4631. [Abstract] [Full Text] [PDF]

				Y Nishizuka Studies and perspectives of protein kinase C Science, July 18, 1986; 233(4761): 305 - 312. [Abstract] [PDF]